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Rampitsch, Christof, Ph.D.

Cereal Proteomics

Contact Information:

195 Dafoe Rd
Winnipeg, Manitoba R3T 2M9
Telephone: 204-983-2385
Fax: 204-983-4604
Email: chris.rampitsch@agr.gc.ca

Areas of Expertise:

  • Enzymes
  • Biochemistry
  • Resistance
  • Disease Organisms
  • Proteins
  • Metabolism
  • Response to Disease and Stress
  • Investigation of plant-pathogen interactions between the fungus Puccinia triticina and wheat using proteomics-based approaches
  • Proteomics and phosphoproteomics of Fusarium graminearum, the causal agent of Fusarium Head Blight in wheat; Application of two-dimensional electrophoresis and mass spectrometry to protein post-translational modification discovery especially phosphorylation during biotic stress signalling.

Current Projects:

  • Proteomic approaches for identifying pathogen and host factors eliciting disease or resistance in the wheat-leaf rust interaction.
  • Identification of phospho-signalling pathways and genomic changes resulting from an overexpressed MEK in wheat.
  • Strategic application of proteomics and plant genotyping toward improved Fusarium Head Blight resistance in Canadian wheat.
  • Redox-signalling in germinating wheat seeds using disulfide-proteomics based approach
  • Proteome and phosphoproteome changes in Fusarium graminearum during mycotoxin production

Affiliations:

  • Adjunct Professor, University of Manitoba
  • Member, Canadian Society of Plant Physiologists

Research publications

2019

2017

2016

2015

2014

2013

2012

2011

2010

2009

  • Rampitsch, C. and Bykova, N.V. (2009). "Methods for Functional Proteomic Analyses.", in Somers, Daryl J. et. Al. (eds.) - Plant Genomics: Methods and Protocols (Methods in Molecular Biology), Human Sciences Press, New York, NY, USA.

Other science related publications

2017

  • Eranthodi A, Subramaniam R, Rampitsch C, González-Peña Fundora D, Goyal RK, Schultz EA, Foroud NA. Characterization of three secreted cysteine-rich proteins from the secretome of Fusarium graminearum. 3rd Lethbridge Research & Development Centre Graduate Symposium. Lethbridge AB, CANADA, November 22, 2017. (Award Winning Presentation) (Oral Presentation)
  • Eranthodi A, Subramaniam R, Rampitsch C, González-Peña Fundora D, Shostak K, Goyal RK, Schultz EA, Foroud NA. The role of three small secreted cysteine-rich proteins of Fusarium graminearum on fungal growth and plant infection. National Fusarium Head Blight Forum. Milwaukee WI, UNITED STATES, December 3-5, 2017. Poster Presentation
  • González-Peña Fundora D, Eranthodi A, Goyal RK, Subramaniam R, Rampitsch C, Thakor N, Foroud NA. Over-expression of a Fusarium graminearum mitogen activated protein kinase, Mgv1. National Fusarium Head Blight Forum. Milwaukee WI, UNITED STATES, December 3-5, 2017. Poster Presentation
  • Gonzàlez-Peña Fundora D, Eranthodi A, Goyal RK, R. Subramaniam, Rampitsch C, Thakor N, Foroud NA. Generation and characterization of a Fusarium graminearum mutant overexpressing the MAPK, Mgv1. 2017 Joint Meeting of the Canadian Phytopathological Society and the Canadian Society of Agronomy. Winnipeg MB, Canada, June 18-22, 2017. Award Winning Presentation.

2016

  • Eranthodi, A., Subramaniam, R., Ouellet, T., Rampitsch, C., Foroud, N.A., and Schultz, E.A. (2016). "Mutant-based screening for pathogen-associated molecular patterns involved in Fusarium head blight disease resistance in wheat.", Prairie University Biology Symposium 2016, The University of Lethbridge, Lethbridge, AB, Canada, February 18-20, 2016. (Poster)
  • Gonzalez-Pena Fundora, D., Thakor, N., Subramaniam, R., Rampitsch, C., and Foroud, N.A. (2016). "Towards the identification of the Mgv1 MAPK pathway in Fusarium graminearum.", Prairie University Biology Symposium 2016, The University of Lethbridge, Lethbridge, AB, Canada, February 18-20, 2016. (Poster)

2015

  • Eranthodi, A., Subramaniam, R., Ouellet, T., Rampitsch, C., Schultz, E.A., and Foroud, N.A. (2015). "Fusarium graminearum mutant screening towards identification of pathogen-associated molecular patterns in the Fusarium head blight-wheat interaction.", 36th Annual Meeting of the Plant Pathology Society of Alberta, Lethbridge, AB, Canada, November 16-18, 2015, Oral Presentation.
  • Eranthodi, A., Subramaniam, R., Ouellet, T., Rampitsch, C., Schultz, E.A., and Foroud, N.A. (2015). "Towards the identification and characterization of pathogen-associated molecular patterns from the Fusarium graminearum secretome.", 1st Lethbridge Research Centre Graduate Student Symposium, Lethbridge, AB, Canada, November 26, 2015, Award Winning: 2nd Place for Oral Presentation.
  • Eranthodi, A., Subramaniam, R., Rampitsch, C., Ouellet, T., Schultz, E.A., and Foroud, N.A. (2015). "Generation of Fusarium graminearum mutants to screen candidate pathogen-associated molecular patterns.", National Fusarium Head Blight Forum, St. Louis, MO, USA, December 6-8, 2015. (Poster)

2014

  • Bakkeren, G., Fellers, J.P., Panwar, V., Joly, D.L., Szabo, L.J., Kolmer, J.A., Hulbert, S., Wang, M., Yin, C., Chen, X., Rampitsch, C., McCallum, B.D., Wang, X., Goldberg, J., Young, S., Hamelin, R.C., Saville, B.J., and Cuomo, C.A. (2014). "Comparative genomics of rust fungi drives research and innovations for crop protection.", 16th conference of the International Society for Molecular Plant-Microbe Interactions, Rhodes, Greece, July 6-10, 2014.
  • Bakkeren, G., Fellers, J.P., Panwar, V., Joly, D.L., Szabo, L.J., Kolmer, J.A., Hulbert, S., Wang, M., Yin, C., Chen, X., Rampitsch, C., McCallum, B.D., Wang, X., Goldberg, J., Young, S., Hamelin, R.C., Saville, B.J., and Cuomo, C.A. (2014). "Comparative genomics of rust fungi drives research and innovations.", XXII Plant & Animal Genome Conference, Town & Country Convention Center, San Diego, CA, USA, January 10-15, 2014.
  • Bykova, N.V., Hu, J.-N., Rampitsch, C., Fan, T., and Radovanovic, N. (2014). "Quantitative comparative proteomic profiling of tissue-specific and membrane-associated seed proteomes in pre-harvest sprouting resistant and susceptible hybrid wheat genotypes.", 2nd Canadian Wheat Symposium, Saskatoon, SK, Canada, June 8-11, 2014. (Poster)
  • Bykova, N.V., Rampitsch, C., and others (2014). "Proteomic approaches to revealing mechanisms of physiological transition from seed dormancy to high germination potential in wheat.", Plant Biology 2014, Portland, Oregon, USA, July 12-16, 2014. (Poster)
  • Ponts, N., Djuric-Ciganovic, S., and Rampitsch, C. (2014). "Analysis of Post-translational Modifications of Histones in Fusarium graminearum.", Canadian Workshop on Tandem Mass Spectrometry, Lake Louise, Canada. (Poster)
  • Rampitsch, C. et al. (2014). "Quantitative proteomics with custom database searching to reveal differences in the haustorial proteomes of three races of wheat leaf rust, Puccinia triticina.", 12th European Conference on Fungal Genetics, Sevilla, Spain. (Poster)
  • Rampitsch, C., Bakkeren, G., and McCallum, B.D. (2014). "Custom database searching to reveal differences in the haustoria proteomes of three races of wheat leaf rust, Puccinia triticina.", 1st Meeting of the International Plant Proteomics Organization, Hamburg, Germany, August 31- September 4, 2014. (Presentation)
  • Rampitsch, C., Beimcik, E., Bakkeren, G., Linning, R., Joly, D.L., and McCallum, B.D. (2014). "Quantitative proteomics with custom database searching to reveal differences in the haustorial proteomes of three races of wheat leaf rust, Puccinia triticina.", 12th European Conference on Fungal Genetics, Sevilla, Spain, March 2014.
  • Rampitsch, C., Beimcik, E., Günel, A., Bakkeren, G., Linning, R., Joly, D.L., McCallum, B.D., and Fan, T. (2014). "Custom database searching to reveal differences in the haustorial proteomes of three races of wheat leaf rust, Puccinia triticina.", Canadian Society of Mass Spectrometry, Montreal, QC, Canada, April 13-16, 2014.

2013

  • Bajracharya, P., McCartney, C., Rampitsch, C., Friesen, T.L., and Daayf, F. (2013). "Purification and characterization of Stagonospora nodorum toxins and mapping of toxin insensitivity.", Plant & Animal Genome XXI Conference, Town & Country Convention Center, San Diego, CA, USA, January 12-16, 2013. (Poster)
  • Bajracharya, P., Rampitsch, C., Daayf, F., and McCartney, C. (2013). "Purification and characterization of Phaeosphaeria nodorum toxins and mapping of toxin insensitivity.", CPS Annual Meeting, Edmonton, AB, Canada. (Poster)
  • Bajracharya, P., Rampitsch, C., Daayf, F., and McCartney, C.A. (2013). "Purification and characterization of Phaeosphaeria nodorum toxins and mapping of toxin insensitivity.", Canadian Phytopathological Society (CPS) & Pacific Division of the American Phytopathological Society Joint 2010 Meeting, University of British Columbia, Vancouver, BC, Canada, June 20-23, 2010. (Poster)
  • Bykova, N.V., Hoehn, B., Rampitsch, C., Hu, J.-N., and Fan, T. (2013). "Redox-mediated regulation of wheat seed dormancy revealed through modification specific proteomics.", 11th International Conference on Reactive Oxygen and Nitrogen Species in Plant, Warsaw, Poland. (Poster)
  • Bykova, N.V., Hu, J.-N., Rampitsch, C., Stebbing, J.A., and Fan, T. (2013). "Proteomic profiling of membrane-associated protein complexes in wheat aleurone tissue using Blue Native gels and label-free semi-quantitative LC-MS/MS approach.", 5th Canadian National Proteomics Network Symposium, Vancouver, BC, Canada. (Poster)
  • Günel, A., Rampitsch, C., Beimcik, E., Fan, T., and Akkaya, M. (2013). "Purification of Stripe Rust Haustoria with monoclonal antibodies.", VIPCA Plant pathogen Interactions, Vienna, Austria. (Poster)
  • Hu, J.-N., Stebbing, J.A., Fan, T., Rampitsch, C., and Bykova, N.V. (2013). "Proteomic analysis reveals changes in abundance, composition, and phosphorylation of membrane-associated protein complexes from wheat aleurone in response to alteration in seed dormancy.", CSPB Annual Meeting, Quebec City, Canada. (Poster)
  • Rampitsch, C., Beimcik, E., Bakkeren, G., and Fan, T. (2013). "Comparative proteomics in Puccinia triticina using race-specific database searches.", 26th CSMS conference, Lake Louise AB, Canada. (Poster)
  • Rampitsch, C., Beimcik, E., Günel, A., Bakkeren, G., McCallum, B.D., and Fan, T. (2013). "Comparative proteomics of monoclonal-antibody enriched haustoria from three races of Puccinia triticina.", 27th American Genetics Society meeting on Fungal Genetics, Asilomar, CA, USA. (Poster)
  • Rampitsch, C., Day, J., Subramaniam, R., and Walkowiak, S. (2013). "Comparative secretome analysis of Fusarium graminearum and two of its non-pathogenic mutants under nitrogen-limitation in vitro.", FHB Satellite Meeting to 27th Fungal Genetics Conference, Asilomar, CA, USA, Oral presentation. (Abstract)
  • Rampitsch, C., Gordon, J.I., and others (2013). "Identification of a novel MADS-box phosphorylation motif by phospho-peptide mapping using CID and ETD.", 26th CSMS conference, Lake Louise AB, Canada. (Poster)
  • Rampitsch, C., Subramaniam, R., Joshi, M., and Fan, T. (2013). "Using redox-proteomics to identify targets of NADPH oxidase-generated reactive oxygen species in Fusarium graminearum.", 12th European Fusarium Seminar, Bordeaux, France, Oral presentation. (Abstract)
  • Simõesa, M., Santos, C., Rampitsch, C., and Lima, N. (2013). "Proteomic analysis of Aspergillus niger type strain (MUM 03.01) after accelerated freeze-drying preservation.", 13th International Conference on Culture Collections, Beijing, China. (Poster)

2012

  • Joshi, M., Subramaniam, R., and Rampitsch, C. (2012). "Identifying targets of NADPH oxidase-mediated redox signalling in Fusarium graminearum using proteomics approaches.", 11th European conference on fugal genetics (ECFG11), Philipps-Universität, Marburg, Germany, March 30-April 2, 2012. (Presentation)
  • Rampitsch, C., Beimcik, E., Günel, A., Gunel, A., Bakkeren, G., McCallum, B.D., and Fan, T. (2012). "The proteome of haustoria from Puccinia triticina.", 13th International Cereal Rusts and Powdery Mildews Conference (ICRPMC), Beijing Friendship Hotel, Beijing, China, August 28-31, 2012. (Poster)

2011

  • Bakkeren, G., Song, X., Panwar, V., Linning, R., Joly, D.L., Wang, X., Rampitsch, C., McCallum, B.D., Fellers, J.P., and Saville, B.J. (2011). "Functional genomic approaches for the wheat leaf rust fungus.", North American Rust Workers Meeting (NARW), St. Paul, MN, USA, June 12, 2011. (Presentation)
  • Hu, J., Hoehn, B., Rampitsch, C., and Bykova, N.V. (2011). "Impact of dormancy genotypes on differential protein expression profiles and redox-sensitive landscape of the proteome in hybrid spring wheat lines.", Plant Canada 2011, Saint Mary’s University, Halifax, NS, Canada, July 17-21, 2011. (Poster)
  • Joshi, M., Subramaniam, R., and Rampitsch, C. (2011). "Identifying targets of NADPH oxidase‐mediated redox signalling in Fusarium graminearum using an LC‐MS approach.", 7th Canadian Workshop on Fusarium Head Blight (CWFHB) / Colloque canadien sur la fusariose, Delta Winnipeg Hotel, Winnipeg, MB, Canada, November 27-30, 2011, pp. 97.
  • Rampitsch, C., Günel, A., Beimcik, E., and Fan, T. (2011). "The proteome of intracellular feeding structures purified from the wheat leaf rust pathogen, Puccinia triticina.", Lake Louise XXIV - 24th Annual Tandem Mass Spectrometry Workshop, Lake Louise, AB, Canada, November 30-December 3, 2011. (Poster)
  • Rampitsch, C., Subramaniam, R., Hoehn, B., Djuric-Ciganovic, S., and Fan, T. (2011). "Phosphoproteomics approach to understand signalling during the Fusarium graminearum-wheat interaction.", 12th International Symposium on Plant Protein Phosphorylation, Tübingen, Germany, September 14-16, 2011. (Poster)

2010

  • Bykova, N.V., Hoehn, B., Rampitsch, C., Hu, J., Stebbing, J.A., and Knox, R.E. (2010). "Thiol redox-sensitive proteome and antioxidant protective mechanisms in wheat dormancy control.", Plant Biology 2010: Joint meeting of the Canadian Society of Plant Physiologists (CSPP) and American Society of Plant Biologists (ASPB), Montréal Convention Centre, Montréal, QC, Canada, July 31-August 4, 2010, P02045. (Abstract)
  • Rampitsch, C., Subramaniam, R., and Fan, T. (2010). "MAP Kinase Mgv1: A Potential Shared Control Point of Butenolide and Deoxynivalenol Biosynthesis in Fusarium Graminearum.", 11th European Fusarium Seminar: Fusarium - Mycotoxins, Taxonomy, Pathogenicity and Host Resistance, Plant Breeding and Acclimatization Institute (IHAR), Radzików, Poland, September 20-23, 2010, OP1.

2009

  • Bakkeren, G., Linning, R., Xu, J., Soltani, B.M., Song, X., Rampitsch, C., Mauthe, W.R., Ching, J., McCallum, B.D., Mulock, B.S., Wang, X., Dickinson, M., Anikster, Y., Eilam, T., Cuomo, C.A., and Fellers, J.P. (2009). "The Dawn of P. triticina Genomics; Searching for Crop-Protection Clues Among Molecular Cereal Rust Fungal Treasure Troves.", Plant & Animal Genome XVII Conference, Town & Country Convention Center, San Diego, CA, USA, January 10-14, 2009, W514.
  • Bakkeren, G., Song, X., Soltani, B.M., Linning, R., Mauthe, W.R., Ching, J., Rampitsch, C., Dickinson, M., Szabo, L.J., Cuomo, C.A., and Fellers, J.P. (2009). "Wheat leaf rust, Puccinia triticina, genome resource building and generation of a protein profile from isolated haustoria.", 14th International Congress on Molecular Plant-Microbe Interactions, Centre des congrès de Québec, Québec City, QC, Canada, July 19-23, 2009.
  • Bykova, N.V., Hoehn, B., Rampitsch, C., Banks, T.W., Stebbing, J.A., Fan, T., and Knox, R.E. (2009). "Redox-sensitive proteome and antioxidant strategies in wheat seed dormancy control.", Meeting on Plant Reactive Oxygen and Nitrogen Species, Helsinki, Finland, July 8-10, 2009, Abstract O15-118. (Presentation)
  • Rampitsch, C., Bakkeren, G., Song, X., Soltani, B.M., Linning, R., Banks, T.W., and Mauthe, W.R. (2009). "A protein profile from in planta infection structures of the wheat leaf rust fungus, Puccinia triticina.", 12th International Cereal Rusts and Powdery Mildews Conference (ICRPMC), Antalya, Turkey, October 13-16, 2009. (Poster)
  • Rampitsch, C., Somers, D.J., Tamburic-Ilincic, L., Fedak, G., Ouellet, T., Humphreys, D.G., and DePauw, R.M. (2009). "Strategic application of proteomics and plant genotyping toward improved FHB resistance in Canadian wheat.", GABI 9th Statusseminar, Potsdam, Germany, March 3-5, 2009. (Poster)
  • Rampitsch, C., Subramaniam, R., Bykova, N.V., and Djuric-Ciganovic, S. (2009). "Protein phosphorylation and DON synthesis: Candidate phosphopeptides from wild-type and map kinase (mgv1)-deficient Fusarium graminearum.", 6th Canadian Workshop on Fusarium Head Blight (CWFHB) / Colloque canadien sur la fusariose, Marriott Ottawa, Ottawa, ON, Canada, November 1-4, 2009, P68. (Abstract)
  • Rampitsch, C., Subramaniam, R., Djuric-Ciganovic, S., and Bykova, N.V. (2009). "The phosphoproteome of Fusarium graminearum at the onset of nitrogen starvation reveals phosphopeptides from proteins with a potential role in mycotoxin regulation.", 11th International Congress on Amino Acids, Peptides and Proteins, Vienna, Austria, August 3-7, 2009, Abstract. Amino Acids 37: 112-113. (Abstract)
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