Sanfaçon, Hélène, Ph.D.
Research Scientist
Contact Information:
4200 Hiaghway 97
PO Box 5000
Summerland, British Columbia V0H 1Z0
Telephone: 250-494-6393
Fax: 250-494-0755
Email: helene.sanfacon@agr.gc.ca
Areas of Expertise:
- molecular biology of plant viruses
- methodology development for plant virus resistance
- characterization of plant virus replication complexes
- characterization of host factors involved in virus replication
- study of viral proteases and design of inhibitors
- characterization of tomato ringspot virus
- development of genetic resistance to plum pox virus
- characterization of viruses infecting fruit trees and small fruits
Current Projects:
- Characterization of replication and translation complexes associated with plant RNA viruses (Sanfacon)
- Development of genetic resistance to Plum pox virus in Canada
Affiliations:
- Adjunct Professor, Dept. Botany, University of British Columbia
- Chair Comoviridae study group, International Committee for the Taxonomy of Viruses
- Associate Editor, Canadian Journal of Botany
Research publications
2019
- Siddell, S.G., Walker, P.J., Lefkowitz, E.J., Mushegian, A.R., Adams, M.J., Dutilh, B.E., Gorbalenya, A.E., Harrach, B., Harrison, R.L., Junglen, S., Knowles, N.J., Kropinski, A.M., Krupovic, M., Kuhn, J.H., Nibert, M., Rubino, L., Sabanadzovic, S., Sanfaçon, H., Simmonds, P., Varsani, A., Zerbini, F.M., Davison, A.J. (2019). Additional changes to taxonomy ratified in a special vote by the International Committee on Taxonomy of Viruses (October 2018), 164(3), 943-946. http://dx.doi.org/10.1007/s00705-018-04136-2
2018
- King, A.M.Q., Lefkowitz, E.J., Mushegian, A.R., Adams, M.J., Dutilh, B.E., Gorbalenya, A.E., Harrach, B., Harrison, R.L., Junglen, S., Knowles, N.J., Kropinski, A.M., Krupovic, M., Kuhn, J.H., Nibert, M.L., Rubino, L., Sabanadzovic, S., Sanfaçon, H., Siddell, S.G., Simmonds, P., Varsani, A., Zerbini, F.M., Davison, A.J. (2018). Changes to taxonomy and the International Code of Virus Classification and Nomenclature ratified by the International Committee on Taxonomy of Viruses (2018), 163(9), 2601-2631. http://dx.doi.org/10.1007/s00705-018-3847-1
- Krupovic M, Blomberg J, Coffin JM, Dasgupta I, Fan H, Geering AD, Gifford R, Harrach B, Hull R, Johnson W, Kreuze JF, Lindemann D, Llorens C, Lockhart B, Mayer J, Muller E, Olszewski NE, Pappu HR, Pooggin MM, Richert-Pöggeler KR, Sabanadzovic S, Sanfaçon H, Schoelz JE, Seal S, Stavolone L, Stoye JP, Teycheney P-Y, Tristem M, Koonin EV, Kuhn JH. 2018. Ortervirales: new virus order unifying five families of reverse-transcribing viruses. J Virol 92:e00515-18. https://doi.org/10.1128/JVI .00515-18.
- Maes, P., Alkhovsky, S.V., Bào, Y., Beer, M., Birkhead, M., Briese, T., Buchmeier, M.J., Calisher, C.H., Charrel, R.N., Choi, I.R., Clegg, C.S., de la Torre, J.C., Delwart, E., DeRisi, J.L., Di Bello, P.L., Di Serio, F., Digiaro, M., Dolja, V.V., Drosten, C., Druciarek, T.Z., Du, J., Ebihara, H., Elbeaino, T., Gergerich, R.C., Gillis, A.N., Gonzalez, J.P.J., Haenni, A.L., Hepojoki, J., Hetzel, U., Hồ, T., Hóng, N., Jain, R.K., Jansen van Vuren, P., Jin, Q., Jonson, M.G., Junglen, S., Keller, K.E., Kemp, A., Kipar, A., Kondov, N.O., Koonin, E.V., Kormelink, R., Korzyukov, Y., Krupovic, M., Lambert, A.J., Laney, A.G., LeBreton, M., Lukashevich, I.S., Marklewitz, M., Markotter, W., Martelli, G.P., Martin, R.R., Mielke-Ehret, N., Mühlbach, H.P., Navarro, B., Ng, T.F.F., Nunes, M.R.T., Palacios, G., Pawęska, J.T., Peters, C.J., Plyusnin, A., Radoshitzky, S.R., Romanowski, V., Salmenperä, P., Salvato, M.S., Sanfaçon, H., Sasaya, T., Schmaljohn, C., Schneider, B.S., Shirako, Y., Siddell, S., Sironen, T.A., Stenglein, M.D., Storm, N., Sudini, H., Tesh, R.B., Tzanetakis, I.E., Uppala, M., Vapalahti, O., Vasilakis, N., Walker, P.J., Wáng, G., Wáng, L., Wáng, Y., Wèi, T., Wiley, M.R., Wolf, Y.I., Wolfe, N.D., Wú, Z., Xú, W., Yang, L., Yāng, Z., Yeh, S.D., Zhāng, Y.Z., Zhèng, Y., Zhou, X., Zhū, C., Zirkel, F., Kuhn, J.H. (2018). Taxonomy of the family Arenaviridae and the order Bunyavirales: update 2018, 163(8), 2295-2310. http://dx.doi.org/10.1007/s00705-018-3843-5
- Mann, K.S., Chisholm, J., Sanfaçon, H. (2019). Strawberry mottle virus (family Secoviridae, order Picornavirales) encodes a novel glutamic protease to process the RNA2 polyprotein at two cleavage sites, 93(5), http://dx.doi.org/10.1128/JVI.01679-18
- Paudel, D.B., Ghoshal, B., Jossey, S., Ludman, M., Fatyol, K., Sanfaçon, H. (2018). Expression and antiviral function of ARGONAUTE 2 in Nicotiana benthamiana plants infected with two isolates of tomato ringspot virus with varying degrees of virulence, 524 127-139. http://dx.doi.org/10.1016/j.virol.2018.08.016
2017
- Adams, M.J., Lefkowitz, E.J., King, A.M.Q., Harrach, B., Harrison, R.L., Knowles, N.J., Kropinski, A.M., Krupovic, M., Kuhn, J.H., Mushegian, A.R., Nibert, M., Sabanadzovic, S., Sanfaçon, H., Siddell, S.G., Simmonds, P., Varsani, A., Zerbini, F.M., Gorbalenya, A.E., Davison, A.J. (2017). Changes to taxonomy and the International Code of Virus Classification and Nomenclature ratified by the International Committee on Taxonomy of Viruses (2017), 162(8), 2505-2538. http://dx.doi.org/10.1007/s00705-017-3358-5
- Adams, M.J., Lefkowitz, E.J., King, A.M.Q., Harrach, B., Harrison, R.L., Knowles, N.J., Kropinski, A.M., Krupovic, M., Kuhn, J.H., Mushegian, A.R., Nibert, M.L., Sabanadzovic, S., Sanfaçon, H., Siddell, S.G., Simmonds, P., Varsani, A., Zerbini, F.M., Orton, R.J., Smith, D.B., Gorbalenya, A.E., Davison, A.J. (2017). 50 years of the International Committee on Taxonomy of Viruses: progress and prospects, 162(5), 1441-1446. http://dx.doi.org/10.1007/s00705-016-3215-y
- Mann, K.S., Walker, M., Sanfaçon, H. (2017). Identification of cleavage sites recognized by the <inf>3</inf>C-like cysteine protease within the two polyproteins of strawberry mottle virus, 8(APR), http://dx.doi.org/10.3389/fmicb.2017.00745
- Sanfaçon, H. (2017). Grand challenge in plant virology: Understanding the impact of plant viruses in model plants, in agricultural crops, and in complex ecosystems, 8(MAY), http://dx.doi.org/10.3389/fmicb.2017.00860
2016
- Adams, M.J., Lefkowitz, E.J., King, A.M.Q., Harrach, B., Harrison, R.L., Knowles, N.J., Kropinski, A.M., Krupovic, M., Kuhn, J.H., Mushegian, A.R., Nibert, M., Sabanadzovic, S., Sanfaçon, H., Siddell, S.G., Simmonds, P., Varsani, A., Zerbini, F.M., Gorbalenya, A.E., Davison, A.J. (2016). Ratification vote on taxonomic proposals to the International Committee on Taxonomy of Viruses (2016), 161(10), 2921-2949. http://dx.doi.org/10.1007/s00705-016-2977-6
- Bhagwat, B., Dickison, V., Ding, X., Walker, M., Bernardy, M., Bouthillier, M., Creelman, A., DeYoung, R., Li, Y., Nie, X., Wang, A., Xiang, Y., Sanfaçon, H. (2016). Genome sequence analysis of five Canadian isolates of strawberry mottle virus reveals extensive intra-species diversity and a longer RNA2 with increased coding capacity compared to a previously characterized European isolate, 161(6), 1657-1663. http://dx.doi.org/10.1007/s00705-016-2799-6
- Fuchs, M., Keichinger-Schmitt, C. and Sanfacon, H. (2017) A renaissance in nepovirus research provides new insights into their molecular interface with hosts and vectors. Adv. Virus Res. 97: 61-105
- Wei, T., Chisholm, J., Sanfaçon, H. (2016). Characterization of a non-canonical signal peptidase cleavage site in a replication protein from tomato ringspot virus, 11(9), http://dx.doi.org/10.1371/journal.pone.0162223
2015
- Adams, M., Lefkowitz, E.J., King, A.M.Q., Bamford, D.H., Breitbart, M., Davison, A.J., Ghabrial, S.A., Gorbalenya, A.E., Knowles, N.J., Krell, P.J., Lavigne, R., Prangishvili, D., Sanfaçon, H., Siddell, S.G., Simmonds, P., and Carstens, E.B. (2015). "Ratification vote on taxonomic proposals to the International Committee on Taxonomy of Viruses (2015).", Archives of Virology, 160(7), pp. 1837-1850. doi : 10.1007/s00705-015-2425-z
- Ghoshal, B. and Sanfaçon, H. (2015). "Symptom recovery in virus-infected plants: revisiting the role of RNA silencing mechanisms.", Virology. doi : 10.1016/j.virol.2015.01.008
- Sanfaçon, H. (2015 in press). "Secoviridae: A family of plant picorna-like viruses with monopartite or bipartite genomes.", - Encyclopedia of Life Sciences, Wiley - Blackwell.
- Sanfaçon, H. (2015). "Plant translation factors and virus resistance.", Viruses, 7(7), pp. 3392-3419. doi : 10.3390/v7072778
- Sanfaçon, H. (2015). "Secoviridae: A family of plant picorna-like viruses with monopartite or bipartite genomes.", - Encyclopedia of Life Sciences, John Wiley & Sons, Ltd.
- Wang, A.M., Tian, L.-N., Brown, D.C.W., Svircev, A.M., Stobbs, L.W., and Sanfaçon, H. (2015). "Generation of efficient resistance to Plum pox virus (PPV) in Nicotiana benthamiana and Prunus domestica expressing triple-intron-spanned double-hairpin RNAs simultaneously targeting 5’ and 3’ conserved genomic regions of PPV.", Acta Horticulturae (ISHS), 1063, pp. 77-84.
2014
- Ghoshal, B., Sanfaçon, H. (2014). Temperature-dependent symptom recovery in Nicotiana benthamiana plants infected with tomato ringspot virus is associated with reduced translation of viral RNA2 and requires ARGONAUTE 1, 456-457(1), 188-197. http://dx.doi.org/10.1016/j.virol.2014.03.026
- Ghoshal, K., Theilmann, J., Reade, R., Sanfacon, H., Rochon, D. (2014). The Cucumber leaf spot virus p25 auxiliary replicase protein binds and modifies the endoplasmic reticulum via N-terminal transmembrane domains, 468-470 36-46. http://dx.doi.org/10.1016/j.virol.2014.07.020
- Karran, R.A., Sanfačon, H. (2014). Tomato ringspot virus coat protein binds to ARGONAUTE 1 and Suppresses the translation repression of a reporter gene, 27(9), 933-943. http://dx.doi.org/10.1094/MPMI-04-14-0099-R
- Walker, M., Chisholm, J., Wei, T., Ghoshal, B., Saeed, H., Rott, M., Sanfaçon, H. (2014). Complete genome sequence of three tomato ringspot virus isolates: evidence for reassortment and recombination, 160(2), 543-547. http://dx.doi.org/10.1007/s00705-014-2240-y
2013
- Laliberte, J-F., Moffett, P., Sanfaçon, H., Wang, A.M., Nelson, R.S., and Schoelz, J.E. (2013). "e-Book on plant virus infection-a cell biology perspective.", Frontiers in Plant Science, 4(Article 203). doi : 10.3389/fpls.2013.00203
- Sanfaçon, H. (2013). "Investigating the role of viral integral membrane proteins in promoting the assembly of nepovirus and comovirus replication factories.", Frontiers in Plant Science, 3(Article 313), pp. 1-7. doi : 10.3389/fpls.2012.00313
- Wang, X., Kohalmi, S.E., Svircev, A., Wang, A., Sanfaçon, H., Tian, L. (2013). Silencing of the Host Factor eIF(iso)4E Gene Confers Plum Pox Virus Resistance in Plum, 8(1), http://dx.doi.org/10.1371/journal.pone.0050627
- Wei, T., Zhang, C., Hou, X., Sanfaçon, H., Wang, A. (2013). The SNARE Protein Syp71 Is Essential for Turnip Mosaic Virus Infection by Mediating Fusion of Virus-Induced Vesicles with Chloroplasts, 9(5), http://dx.doi.org/10.1371/journal.ppat.1003378
- Wetzel, T., Chisholm, J., Dupuis-Maguiraga, L., Bassler, A., Sanfacon, H. (2013). In vitro and in vivo evidence for differences in the protease activity of two arabis mosaic nepovirus isolates and their impact on the infectivity of chimeric cDNA clones, 446(1-2), 102-111. http://dx.doi.org/10.1016/j.virol.2013.07.040
2011
- Jovel, J., Walker, M., Sanfaçon, H. (2011). Salicylic acid-dependent restriction of tomato ringspot virus spread in tobacco is accompanied by a hypersensitive response, local RNA silencing, and moderate systemic resistance, 24(6), 706-718. http://dx.doi.org/10.1094/MPMI-09-10-0224
2010
- Laliberte, J-F. and Sanfaçon, H. (2010). "Cellular remodeling during plant virus infection.", Annual Review of Phytopathology, 48, pp. 69-91. doi : 10.1146/annurev-phyto-073009-114239
- Tian, L.-N., Svircev, A.M., Sanfaçon, H., Brown, D.C.W., Schneider, K.E., Sibbald, S., and Malutan, T. (2010). "Development of Plum pox virus resistance in plum via expression of an intron-spliced hairpin HC-Pro transgene.", Genes, Genomes & Genomics, 4(Suppl. 1), pp. 28-31.
2009
- Jafarpour, B., Sanfaçon, H. (2009). Insertion of large amino acid repeats and point mutations contribute to a high degree of sequence diversity in the X4 protein of tomato ringspot virus (genus Nepovirus), 154(10), 1713-1717. http://dx.doi.org/10.1007/s00705-009-0497-3
- Sanfaçon, H. (2009). "Secoviridae: the amalgamation of the family Comoviridae and Sequiviridae.", Encyclopedia of Life Sciences, eLS. doi : 10.1002/9780470015902.a0000764.pub2
- Sanfaçon, H., Wellink, J., Le Gall, O., Karasev, A., Van Der Vlugt, R., Wetzel, T. (2009). Secoviridae: A proposed family of plant viruses within the order Picornavirales that combines the families Sequiviridae and Comoviridae, the unassigned genera Cheravirus and Sadwavirus, and the proposed genus Torradovirus, 154(5), 899-907. http://dx.doi.org/10.1007/s00705-009-0367-z
- Wang, A.M., Tian, L.-N., Huang, T.-S., Brown, D.C.W., Svircev, A.M., Stobbs, L.W., Miki, B.L.A., and Sanfaçon, H. (2009). "The development of genetic resistance to plum pox virus in transgenic Nicotiana benthamiana and Prunus domesticus.", Acta Horticulturae (ISHS), 839, pp. 665-672.
Other science related publications
2015
- Wei, T., Chisholm, J., and Sanfaçon, H. (2015). "Cleavage of tomato ringspot virus NTP-binding protein by the membrane-associated signal peptidase.", 34th Annual Meeting of American Society for Virology, London, Ontario, Canada, July 11-15, 2015. (Abstract)
2014
- Ghoshal, B. and Sanfaçon, H. (2014). "Temperature-dependent symptom recovery in Nicotiana benthamiana plants infected with tomato ringspot virus is associated with reduced translation of viral RNA2 and requires ARGONAUTE 1.", International Union of Microbiological Societies Congress (IUMS 2014), Montréal, QC, Canada, July 27-August 1, 2014. (Poster)
- Sanfaçon, H. (2014). "Updates to the Plant Virus Taxonomy.", International Union of Microbiological Societies Congresses, Montreal, QC, Canada, July 27-August 1, 2014. (Poster)
- Wei, T. and Sanfaçon, H. (2014). "Evidence for in vitro and in vivo cleavage of the C-terminal region of the tomato ringspot virus NTP-binding protein by the membrane-associated signal peptidase.", International Union of Microbiological Societies Congress (IUMS 2014), Montréal, QC, Canada, July 27-August 1, 2014. (Poster)
2013
- Ghoshal, B. and Sanfaçon, H. (2013). "Symptom recovery in Nicotiana benthamiana plants infected with tomato ringspot virus is associated with Argonaute 1-dependent translational repression of the viral genome.", Keystone Symposium: RNA Silencing, Whistler Conference Centre, Whistler, BC, Canada, March 19-24, 2013. (Poster)
- Karran, R., Walker, M.C., and Sanfaçon, H. (2013). "Tomato ringspot virus coat protein as a potential translational enhancer.", 32nd Annual Meeting of the American Society for Virology, Pennsylvania State University, University Park, Pennsylvania, USA, July 20-24, 2013, (oral presentation).
- Stobbs, L.W., Hunter, D.M., Samara, R., Lowery, T., Lowery, D.T., and Sanfaçon, H. (2013). "Progress in Epidemiological Research on Plum Pox Virus.", The Tender Fruit Grape Vine (for Commercial Fruit Growers), 17(5), pp. 5-6. (Newsletter)
- Tian, L.-N., Wang, X., Svircev, A.M., Wang, A.M., Sanfaçon, H., Brown, D.C.W., Kohalmi, S.E., and Lu, M. (2013). "Development of Plum pox virus resistance in plum grown in Canada via plant biotechnology.", 2nd International Symposium on Plum Pox Virus, Olomouc, Czech Republic, September 3-6, 2013.
- Wang, A.M., Elhiti, M., Wang, H., Li, Y., Tian, L.-N., Svircev, A.M., Sanfaçon, H., and Brown, D.C.W. (2013). "Development of genetic resistance to Plum pox virus in Canada.", 2nd International Symposium on Plum Pox Virus, Olomouc, Czech Republic, September 3-6, 2013.
- Wei, T. and Sanfaçon, H. (2013). "Functional characterization of full-length and truncated forms of the tomato ringspot virus RNA-dependent RNA polymerase.", 32nd Annual Meeting of the American Society for Virology, Pennsylvania State University, University Park, Pennsylvania, USA, July 20-24, 2013. (Poster)
- Wetzel, T., Chisholm, J., Dupuis-Maguiraga, L., Bassler, A., and Sanfaçon, H. (2013). "Influence of the protease and polyprotein cleavage efficiency on the infectivity of chimeric Arabis mosaic nepovirus cDNA clones.", Keystone symposium on positive-strand RNA viruses, Boston, Massachussetts, USA, April 28-May 3, 2013. (Poster)
2012
- Sanfaçon, H., Walker, M.C., Jovel, J., Karran, R., Ghoshal, B., Chisholm, J., and Wei, T. (2012). "In depth study of the interaction of peach viruses with plant defense responses for improved management of PPV.", Joint Canadian Phytopathological Society (CPS) 83rd Annual Meeting and International PPV Meeting, Marriott Gateway On The Falls, Niagara Falls, ON, Canada, June 24-27, 2012. (Presentation)
- Tian, L.-N., Wang, X., Kohalmi, S.E., Svircev, A.M., Wang, A.M., Sanfaçon, H., Brown, D.C.W., and Schneider, K.E. (2012). "Control of the expression of the host factor eIF(iso)4E gene for Plum Pox Virus resistance.", Joint Canadian Phytopathological Society (CPS) 83rd Annual Meeting and International PPV Meeting, Marriott Gateway On The Falls, Niagara Falls, ON, Canada, June 24-27, 2012. (Presentation)
- Wei, T., Chisholm, J., and Sanfaçon, H. (2012). "Stability of the tomato ringspot virus RNA-dependent RNA polymerase in plants: exploring possible interactions with components of the proteaseome and with caspase-like proteases.", XV International Congress of Virology, Sapporo, Japan, September 11-16, 2011. (Abstract)
2011
- Ghoshal, B. and Sanfaçon, H. (2011). "Accumulation of viral products during the symptomatic and recovery phase in Nicotiana benthamiana plants infected with tomato ringspot virus isolates differing in their virulence.", XV International Congress of Virology, Sapporo, Japan, September 11-16, 2011. (Presentation)
- Sanfaçon, H. and Fuchs, M. (2011). "Tomato ringspot virus.", in Hadidi, A., Barba, M., Candresse, T., and Jelkmann, W. (eds.) - Virus and Virus-Like Diseases of Pome and Stone Fruits, American Phytopathological Society (APS), Chapter 9, Part II. Pome Fruit Viruses and Related Diseases.
- Sanfaçon, H., Gorbalenya, A.E., Knowles, N.J., and Chen, Y. (2011). "Order Picornavirales.", in King, A.M.Q., Adams, M.J., Carstens, E.B. and Lefkowitz, E.J. (eds.) - Virus Taxonomy: Ninth Report of the International Committee on Taxonomy of Viruses, Elsevier, pp. 835-839.
- Sanfaçon, H., Iwanami, T., Karasev, A.V., van der Vlugt, R., Wellink, J., Wetzel, T., and Yoshikawa, N. (2011). "Family Secoviridae.", in King, A.M.Q., Adams, M.J., Carstens, E.B. and Lefkowitz, E.J. (eds.) - Virus Taxonomy: Ninth Report of the International Committee on Taxonomy of Viruses, Elsevier, pp. 881-899.
- Wei, T., Chisholm, J., and Sanfaçon, H. (2011). "Sequence analysis of RNA1-encoded replication proteins of tomato ringspot nepovirus isolates.", XV International Congress of Virology, Sapporo, Japan, September 11-16, 2011. (Poster)
2010
- Brown, D.C.W., Wang, A.M., Tian, L.-N., Svircev, A.M., Sanfaçon, H., and Miki, B.L.A. (2010 Internal). Developmnt of genetic resistance to PPV in Prunus Sp. Research Report to the Plum Pox Virus Working Group, St. Catherines, Ontario, January 26-28, 2010. (Report)
- Sanfaçon, H. (2010). "Emerging technologies for the control of plant virus diseases.", in Wang, A. (ed.) - Principles and Practice of Advanced Technology in Plant Virology, Research Signpost, Kerala, India, Chapter 17, pp. 295-310.
- Tian, L.-N., Svircev, A.M., Sanfaçon, H., and Brown, D.C.W. (2010). "Plum pox virus resistance stability and expression of specific siRNAs in plum.", US/Canada Plum Pox Virus Update and International Research Symposium, Kearneysville, WV, USA, January 4-6, 2010.
2009
- Brown, D.C.W., Wang, A.M., Tian, L.-N., Svircev, A.M., Sanfaçon, H., and Miki, B.L.A. (2009 Internal). Developing genetic resistance to Plum Pox Virus. Research Report to the Plum Pox Virus Working Group, St Catherines, Ontario, January 27, 2009. (Report)
- Le Gall, O., Sanfaçon, H., Ikegami, M., Iwanami, T., Jones, T., Karasev, A.V., Lehto, K., Wellink, J., Wetzel, T., and Yoshikawa, N. (2009). "Cheravirus and Sadwavirus: Two unassigned genera of plant positive-sense single-stranded RNA viruses formerly considered atypical members of the genus Nepovirus (family Comoviridae) [Archives of Virology 152(9), pp.1767-1774] (Erratum).", Archives of Virology, 154(6), pp. 1019.
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