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Agriculture and Agri Food Canada Biopesticide: Pseudomonas fluorescens BRG100

Soil bacteria as pre-emergent bioherbicides for annual grass weeds

Green foxtail and wild oat represent two major annual grass weeds in crops such as cereals, canola, and pulses. It has been estimated that it costs farmers more than $4 million annually to control herbicide-resistant wild oat and green foxtail. Biological control is one more tool in the weed management toolbox for farmers to use.

Pseudomonas fluorescens strain BRG100 - in petri dish and under microscope

Biological control of weeds with microorganisms represents the next generation of pesticides. Soil bacteria that colonize plant roots are being developed as pre-emergent biocontrol agents of green foxtail and wild oat. The bacteria inhibit weed seed germination and suppress root growth. The leading bacterial candidate is Pseudomonas fluorescens strain BRG100.

Pseudomonas fluorescens strain BRG100 - Control
Control
Pseudomonas fluorescens strain BRG100 - Treated with bacteria
Treated with bacteria

BRG100 can provide up to 85 to 90% weed control in the field and it has been shown to control herbicide-resistant green foxtail and wild oat populations.

Thriving untreated green foxtail weeds.
Green Foxtail - untreated
Failing green foxtail weeds treated with BRG100.
Green Foxtail - BRG100

Manufacturing: How do we mass-produce and formulate the bacteria into ‘pesta’ granules?

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1) Fermentation
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2) Mixing and blending
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3) Extrusion
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4) Spheronization
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5) Fluidized bed drying
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6) Pesta granules

Delivery of the formulated bacteria

Farmers with a tractor pulling standard seeding equipment.

Pesta granules containing the BRG100 are applied with standard seeding equipment.

Pesta granules containing the BRG100.

Wheat field treated with BRG100

Untreated wheat field
Untreated
Wheat field treated with BRG100 - Low-rate
Low-rate
Wheat field treated with BRG100 - High-rate
High-rate
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