Language selection

Search

Errampalli, D. 2017. DNA Sequence-based Identification of Fungi from Ginseng Roots and Soils.

Errampalli, D. 2017. DNA Sequence-based Identification of Fungi from Ginseng Roots and Soils. Presented at the Plant Biology 2017 conference in Honolulu, Hi, USA from 24-29, 2017. (abstract)

Abstract

American ginseng root is highly valued for its health benefits. Replant disease causes severe yield losses in ginseng. Detection and identification of pathogens are the first critical steps in developing disease control. The goal of this study was to determine whether sequence analysis of internal transcribed spacer region (ITS) can be used to detect and identify fungal pathogens in soils in which the ginseng was grown and symptomatic ginseng roots. Soils were collected from gardens (fields) with and without replant disease (control) in 2014. Ginseng roots with symptoms were collected from three ginseng gardens in Ontario. Fungi were isolated from soils and root samples on different selective media. Genomic DNA was extracted from each of the fungal isolates and the ITS region was amplified by PCR with ITS1 and ITS4 primers. The amplified DNA was sequenced and aligned against sequences in GenBank. Sequence based identification was carried out on 109 fungal isolates. The following fungi were most frequent and percentage of isolates is given in parenthesis. Fusarium oxysporum (44%), Fusarium solani (14.8%), and Cladosporium cladosporoides (16.0%) and Fusarium species (13%) were from 55 isolates from the soils in which ginseng was cultivated, b) Bionectria sp./ Clonostachys sp. (27.3%), Cylindrocarpon (13.6%), and Mortierella sp., (13.6%) were from 22 isolates from the soils around wild ginseng plants ,and c) F. solani (46%), Epicoccum nigram (12.5%), Pseudallescheria boydii / Scedosporium minutisporum (9.4%) were from 32 isolates from the symptomatic ginseng roots. Some of these isolates were found to be pathogenic to ginseng roots. This information is important for determining the complex that causes ginseng replant disease.

Report a problem on this page
Please select all that apply:
Date modified: