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Haplotype analysis for Fusarium head blight resistance in a collection of Brazilian spring wheat

Langille L, Voldeng H, Wang X, Khanizadeh S, McCartney C, Humphreys G (2016) Haplotype analysis for Fusarium head blight resistance in a collection of Brazilian spring wheat. Proc 8th Can Workshop on Fusarium Head Blight, Ottawa, Nov 20-22, P33.

Abstract

Genetic variability is a crucial element in any wheat breeding program. In 2014, 81 wheat lines from various Brazilian breeding institutions were received as part of a Canada-Brazil germplasm exchange. It was anticipated that the material would provide a valuable new source of disease resistance, particularly for Fusarium head blight (FHB) and leaf rust. Seventy-nine of the Brazilian lines with spring wheat habit were genotyped using DNA markers (SSR, STS, and KASP) at three important FHB quantitative trait loci (QTL). These QTL were previously reported on chromosomes 3BS, 5AS and 6BS respectively, in cultivar Sumai3. In addition, lines were genotyped for KASP and SSR markers at three reduced-height genes (Rht-B1, Rht-D1 and Rht8). Visual Ratings Index (VRI) for reactions to FHB were evaluated in the Ottawa inoculated nursery in 2015 and 2016, and deoxynivalenol (DON) content was determined from 2015 harvested nursery samples. The Brazilian collection does not possess the Sumai3 haplotype for FHB resistance QTL on 3BS (Fhb1) or 6BS (Fhb2). While 63% of the lines amplified the Sumai3 allele at gwm415 on 5AS, none amplified the Sumai3 allele at adjacent SSRs. Nevertheless, VRI ranged from 2.0 to 95.0 in 2015 and 13.3 to 95.0 in 2016. Similarly, DON levels ranged from 1.3 to 35.0 ppm. The Brazilian collection appears to possess FHB resistance not associated with the three major Sumai3 FHB QTL. A significant (P<0.05) negative correlation between plant height and VRI was found but no significant relationship between plant height and DON was observed.

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