Whole plant regeneration of common bean (Phaseolus vulgaris) varieties.
Many Phaseolus vulgaris varieties are recalcitrant to regeneration. This is due to several factors including shooting and rooting abilities and differences in phenolic content that can either delay or restrict full plant regeneration. A recent study by Collado et al. (2013, Scientia Horticulturae 135:109) has shown successful regeneration of germinated seeds in few common bean varieties. Using a modified version of this protocol, we tested its efficiency on 27 different common bean varieties using cotyledon and embryonic axis tissues. There was variability between cultivars and tissues in callus production, shoot regeneration, and then rooting. From the initially tested varieties, only a few produced both shoots and roots with successful potting of four varieties (Pinto and Red Kidney bean market classes). Downstream goals of this project are to apply efficient tissue culture techniques in common bean to developing herbicide resistant cultivars for Canadian agriculture in combination with genome editing.
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