Using yeasts to study mycotoxin toxicity and interactions.
Gleddie, S., Hermans, A., Gunter, A., Bahadoor, A., Harris, L.J. Using yeasts to study mycotoxin toxicity and interactions. Book of Abstracts of Lectures and Posters, Session 10, World Mycotoxin Forum, Winnipeg, MB, June 6-9, 2016, p. 69.
Yeast species are ideal model organisms to study the toxicity, mode of actions, cellular interactions and effects of mycotoxins. As a well characterized model eukaryotic organism, yeast (Saccharomyces cerevisiae) has many advantages over more complex multicellular models. Yeast has a sequenced genome with gene deletion collections and tagged protein expression collections. They have been adapted for two-hybrid interactome analysis, while gene replacement methods abound. These extensive genomic and proteomic resources permit rapid development of real time assays to study the effects and consequences of toxins both singly, and in combinations as well as fungal filtrates or secretomes. We have conducted numerous studies to measure the effects of single amino acid substitutions in a protein thought to be the target of mycotoxins (ribosomal protein L3). We will highlight results from studies using 1) novel antibodies directed against mycotoxins, 2) the yeast knock-out collection to search for novel targets of mycotoxins, and 3) yeast with modified transporter capabilities to identify and alter cellular exporters of mycotoxins.
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