Validation and diagnostic marker development for a genetic region associated with wheat yellow mosaic virus resistance
Xiao, J., Chen, X., Xu, Z., Guo, J., Wu, Z., Wang, H., Zhu, X., Nie, M., Bie, T., Cheng, S., Zhu, T.T., Luo, M., You, F.M., Wang, X. (2016). Validation and diagnostic marker development for a genetic region associated with wheat yellow mosaic virus resistance, 211(1), 91-101. http://dx.doi.org/10.1007/s10681-016-1731-1
© 2016, Springer Science+Business Media Dordrecht. Wheat yellow mosaic disease (WYM) became more serious in China in the recent years. Cultivation of resistant varieties is the major strategy for disease control. To identify WYM resistant genes in Chinese wheat cultivar ‘Yining Xiaomai’, a population of 106 recombinant inbred lines (RIL) from a cross between Yining Xiaomai and Zhen 9523 (WYM-susceptible) was generated. Phenotypic data of WYM resistance of the RILs were collected over three successive years in a naturally infected field nursery. A linkage map consisting of 29 genetic linkage groups involving 144 SSR or EST markers was constructed, using the JoinMap 4.0 software. By composite interval mapping using IciMapping 4.0, a stable major QTL associated with WYM resistance, designated QYm.nau-2D, was mapped to chromosome 2D which explained up to 93.2 % of the phenotypic variance. More linkage markers including 11 SNP and 11 STS markers were developed to saturate the QYm.nau-2D region. The presence of WYM resistance was further validated using a resistant RIL derived F2 population consisting 318 lines. A high resolution map at QYm.nau-2D region was re-constructed which established a basis for map-based cloning of the resistant gene. Four markers were validated to be diagnostic markers to discriminate 195 breeding lines with known pedigree. These markers could be used in marker-assisted selection to breeding WYM resistance.
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