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Alteration of the microbiota and virulence gene expression in E. Coli O157:H7 in pig ligated intestine with and without AE lesions

Liu, B., Yin, X., Yu, H., Feng, Y., Ying, X., Gong, J., Gyles, C.L. (2015). Alteration of the microbiota and virulence gene expression in E. Coli O157:H7 in pig ligated intestine with and without AE lesions, 10(6), http://dx.doi.org/10.1371/journal.pone.0130272

Abstract

Copyright © 2015 Liu et al. Background: Previously we found that E. coli O157:H7 inoculated into ligated pig intestine formed attaching and effacing (AE) lesions in some pigs but not in others. The present study evaluated changes in the microbial community and in virulence gene expression in E. coli O157:H7 in ligated pig intestine in which the bacteria formed AE lesions or failed to form AE lesions. Methodology/Principal Findings: The intestinalmicrobiota was assessed by RNA-based denaturing gradient gel electrophoresis (DGGE) analysis. The DGGE banding patterns showed distinct differences involving two bands which had increased intensity specifically in AE-negative pigs (AE- bands) and several bands which were more abundant in AE-positive pigs. Sequence analysis revealed that the two AE- bands belonged to Veillonella caviae, a species with probiotic properties, and Bacteroides sp. Concurrent with the differences inmicrobiota, gene expression analysis by quantitative PCR showed that, compared with AE negative pigs, E. coliO157:H7 in AE positive pigs had upregulated genes for putative adhesins, non-LEE encoded nleA and quorum sensing qseF, acid resistance gene ureD, and genes fromthe locus of enterocyte effacement (LEE). Conclusions/Significance: The present study demonstrated that AE-positive pigs had reduced activities or populations of Veillonella caviae and Bacterioides sp. compared with AE-negative pigs. Further studies are required to understand how the microbiota was changed and the role of these organisms in the control of E. coli O157:H7.

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