An epimer of deoxynivalenol: purification and structure identification of 3-epi-deoxynivalenol
He, J.W., Yang, R., Zhou, T., Boland, G.J., Scott, P.M., Bondy, G.S. (2015). An epimer of deoxynivalenol: purification and structure identification of 3-epi-deoxynivalenol, 32(9), 1523-1530. http://dx.doi.org/10.1080/19440049.2015.1072771
© Her Majesty the Queen in Right of Canada, as represented by the Minister of Agriculture and Agri-Food Canada. In an investigation of deoxynivalenol (DON)-transformation products by Devosia mutans 17-2-E-8, the major product was identified as 3-epi-DON. This DON-transformation product was analysed by liquid chromatography and identified by congruent retention time and UV/Vis spectrum, as well as mass spectrometric data. Nuclear magnetic resonance (NMR) experiments including correlation spectroscopy (COSY), heteronuclear single quantum coherence (HSQC) and nuclear overhauser effect (NOE) were conducted for structural characterisation of 3-epi-DON. High-speed counter-current chromatography (HSCCC) was applied to scale up the separation of 3-epi-DON from DON in a D. mutans 17-2-E-8 culture. From the culture where 100 mg DON was applied, 56 mg of 3-epi-DON (purity of 96.8%) was obtained from the HSCCC. The purified 3-epi-DON will be used for toxicological characterisation studies of this chemical.
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