A landscape of hairy and twisted: Hunting for new trichome mutants in the Saskatoon Arabidopsis T-DNA population
Taheri, A., Gao, P., Yu, M., Cui, D., Regan, S., Parkin, I., Gruber, M. (2015). A landscape of hairy and twisted: Hunting for new trichome mutants in the Saskatoon Arabidopsis T-DNA population, 17(2), 384-394. http://dx.doi.org/10.1111/plb.12230
© 2014 German Botanical Society and The Royal Botanical Society of the Netherlands. A total of 88 new Arabidopsis lines with trichome variation were recovered by screening 49,200 single-seed descent T3 lines from the SK activation-tagged population and from a new 20,000-line T-DNA insertion population (called pAG). Trichome variant lines were classified into 12 distinct phenotype categories. Single or multiple T-DNA insertion sites were identified for 89% of these mutant lines. Alleles of the well-known trichome genes TRY, GL2 and TTG1 were recovered with atypical phenotype variation not reported previously. Moreover, atypical gene expression profiles were documented for two additional mutants specifying TRY and GL2 disruptions. In remaining mutants, ten lines were disrupted in genes coding for proteins not implicated in trichome development, five were disrupted in hypothetical proteins and 11 were disrupted in proteins with unknown function. The collection represents new opportunities for the plant biology community to define trichome development more precisely and to refine the function of individual trichome genes.
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