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Strategies and approaches to propagate strawberry nuclear stocks using a bioreactor.

Debnath, S.C. (2014). "Strategies and approaches to propagate strawberry nuclear stocks using a bioreactor.", Acta Horticulturae (ISHS), 1049, pp. 145-150.


The important role of dietary fruits in maintaining human health has led to a dramatic increase of the global berry crop production. While strawberries have long been enjoyed huge popularity among consumers, tremendous progress in plant tissue culture, resulting in great advances in micropropagation, has occurred. The in vitro morphogenesis seems to be highly dependent on plant growth regulators and media used for culture, which is again genotype specific. Although automation of micropropagation in bioreactors has been advanced as a possible way of reducing propagation cost, optimal plant production depends upon better understanding of physiological and biochemical responses of plant to the signals of culture microenvironment and an optimization of specific physical and chemical culture conditions to control the morphogenesis of berry plants in liquid culture systems. Clonal fidelity can be a serious problem and molecular strategies have been developed in order to reduce the variation to manageable levels. The paper describes the progress in-depth of various aspects of strawberry propagation in vitro in liquid media using bioreactors, for their improvement and for commercial production. The paper also focuses on the employment of molecular markers in micropropagated plants for the assessment of genetic fidelity, uniformity, stability and trueness-to-type among donor plants and tissue culture regenerants.

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