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Strategies to propagate Vaccinium nuclear stock and use of molecular markers for clonal fidelity.

Debnath, S.C. (2014). "Strategies to propagate Vaccinium nuclear stock and use of molecular markers for clonal fidelity.", Acta Horticulturae (ISHS), 1017, pp. 93-99.


Significant progress in plant tissue culture, resulting in great advances in micropropagation, has occurred with Vaccinium species. The in vitro morphogenesis is highly dependent on plant growth regulators and media used for culture, which is again genotype specific. Although automation of micropropagation in bioreactors has been advanced as a possible way of reducing propagation cost, optimal plant production depends upon better understanding of physiological and biochemical responses of plants to the signals of culture microenvironment and an optimization of specific physical and chemical culture conditions to control the morphogenesis of berry plants in liquid culture systems. Increased branching, vigorous vegetative growth and change in biochemical components are noted in micropropagated Vaccinium plants acclimatized under ex vitro condition. Clonal fidelity can be a serious problem and strategies have been developed in order to reduce the variation to manageable levels. Molecular markers have been introduced in tissue culture research and can potentially be used in various facets of pertinent studies with berry crops. This review describes the progress in-depth of various aspects of Vaccinium culture in vitro, characterization of micropropagated plants for morphological characters, anthocyanin contents and antioxidant activity, and on the employment of molecular markers in these plants for the assessment of genetic fidelity, uniformity, stability and trueness-to-type among donor plants and tissue culture regenerants.

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