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Genomic relationships among sixteen species of Avena based on (ACT)<inf>6</inf> trinucleotide repeat FISH

Luo, X., Tinker, N.A., Zhou, Y., Wight, C.P., Liu, J., Wan, W., Chen, L., Peng, Y. (2018). Genomic relationships among sixteen species of Avena based on (ACT)<inf>6</inf> trinucleotide repeat FISH, 61(1), 63-70. http://dx.doi.org/10.1139/gen-2017-0132

Abstract

© 2018 Published by NRC Research Press. Knowledge of the locations of repeat elements could be very important in the assembly of genome sequences and their assignment to physical chromosomes. Genomic and species relationships among 16 species were investigated using fluorescence in situ hybridization (FISH) with the Am1 and (ACT)6 probes. The Am1 oligonucleotide probe was particularly enriched in the C genomes, whereas the (ACT)6 trinucleotide repeat probe showed a diverse distribution of hybridization patterns in the A, AB, C, AC, and ACD genomes but might not be present in the B and D genomes. The hybridization pattern of Avena sativa was very similar to that of A. insularis, indicating that this species most likely originated from A. insularis as a tetraploid ancestor. Although the two FISH probes failed to identify relationships of more species, this proof-of-concept approach opens the way to the use of FISH probes in assigning other signature elements from genomic sequence to physical chromosomes.

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