A role for seed storage proteins in Arabidopsis seed longevity
Nguyen, T.P., Cueff, G., Hegedus, D.D., Rajjou, L., Bentsink, L. (2015). A role for seed storage proteins in Arabidopsis seed longevity, 66(20), 6399-6413. http://dx.doi.org/10.1093/jxb/erv348
© 2015 The Author. Published by Oxford University Press on behalf of the Society for Experimental Biology. Proteomics approaches have been a useful tool for determining the biological roles and functions of individual proteins and identifying the molecular mechanisms that govern seed germination, vigour and viability in response to ageing. In this work the dry seed proteome of four Arabidopsis thaliana genotypes, that carry introgression fragments at the position of seed longevity quantitative trait loci and as a result display different levels of seed longevity, was investigated. Seeds at two physiological states, after-ripened seeds that had the full germination ability and aged (stored) seeds of which the germination ability was severely reduced, were compared. Aged dry seed proteomes were markedly different from the after-ripened and refected the seed longevity level of the four genotypes, despite the fact that dry seeds are metabolically quiescent. Results confrmed the role of antioxidant systems, notably vitamin E, and indicated that protection and maintenance of the translation machinery and energy pathways are essential for seed longevity. Moreover, a new role for seed storage proteins (SSPs) was identifed in dry seeds during ageing. Cruciferins (CRUs) are the most abundant SSPs in Arabidopsis and seeds of a triple mutant for three CRU isoforms (crua crub cruc) were more sensitive to artifcial ageing and their seed proteins were highly oxidized compared with wild-type seeds. These results confrm that oxidation is involved in seed deterioration and that SSPs buffer the seed from oxida-tive stress, thus protecting important proteins required for seed germination and seedling formation.
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