A new method for studying population genetics of cyst nematodes based on Pool-Seq and genomewide allele frequency analysis
Mimee, B., Duceppe, M.O., Véronneau, P.Y., Lafond-Lapalme, J., Jean, M., Belzile, F., Bélair, G. (2015). A new method for studying population genetics of cyst nematodes based on Pool-Seq and genomewide allele frequency analysis, 15(6), 1356-1365. http://dx.doi.org/10.1111/1755-0998.12412
© 2015 John Wiley & Sons Ltd. Cyst nematodes are important agricultural pests responsible for billions of dollars of losses each year. Plant resistance is the most effective management tool, but it requires a close monitoring of population genetics. Current technologies for pathotyping and genotyping cyst nematodes are time-consuming, expensive and imprecise. In this study, we capitalized on the reproduction mode of cyst nematodes to develop a simple population genetic analysis pipeline based on genotyping-by-sequencing and Pool-Seq. This method yielded thousands of SNPs and allowed us to study the relationships between populations of different origins or pathotypes. Validation of the method on well-characterized populations also demonstrated that it was a powerful and accurate tool for population genetics. The genomewide allele frequencies of 23 populations of golden nematode, from nine countries and representing the five known pathotypes, were compared. A clear separation of the pathotypes and fine genetic relationships between and among global populations were obtained using this method. In addition to being powerful, this tool has proven to be very time- and cost-efficient and could be applied to other cyst nematode species.
Report a problem on this page
- Date modified: