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Characterisation of a novel water-soluble polysaccharide from Leuconostoc citreum SK24.002

Miao, M., Bai, A., Jiang, B., Song, Y., Cui, S.W., Zhang, T. (2014). Characterisation of a novel water-soluble polysaccharide from Leuconostoc citreum SK24.002, 36 265-272. http://dx.doi.org/10.1016/j.foodhyd.2013.10.014

Abstract

Water-soluble polysaccharides that are produced from sucrose by lactobacillus strains isolated from Chinese traditional pickled vegetables were investigated. Strain SK24.002, which secreted an exopolysaccharide with a high dextranase resistance, was identified as Leuconostoc citreum via 16S rDNA sequence analysis. A polysaccharide yield of 35g/L was achieved at 20h in Man-Rogosa-Sharpe medium containing 100g/L sucrose. The water-soluble polysaccharide consisted exclusively of glucose, and the weight-average molecular weight was 4.62×107Da. Spectroscopy results demonstrated that the water-soluble exopolysaccharide was mainly composed of α-1,3 and α-1,6 linked d-glucopyranose units. Methylation analysis revealed that the ratio of α-1,6 linkages to α-1,3 linkages was 5:4. These results suggested a possible molecular structure for the water-soluble exopolysaccharide that consists of a backbone chain of alternating α-1,3 and α-1,6 linkages with a branched point at the C6 of the 1,3,6-linked d-glucopyranose unit. This novel polysaccharide may have applications as a functional ingredient in processed foods. © 2013 .

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