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Proteomic profiling reveals insights into Triticeae stigma development and function

Nazemof, N., Couroux, P., Rampitsch, C., Xing, T., Robert, L.S. (2014). Proteomic profiling reveals insights into Triticeae stigma development and function, 65(20), 6069-6080. http://dx.doi.org/10.1093/jxb/eru350

Abstract

© 2014 The Author. Published by Oxford University Press on behalf of the Society for Experimental Biology. To our knowledge, this study represents the first high-throughput characterization of a stigma proteome in the Triticeae. A total of 2184 triticale mature stigma proteins were identified using three different gel-based approaches combined with mass spectrometry. The great majority of these proteins are described in a Triticeae stigma for the first time. These results revealed many proteins likely to play important roles in stigma development and pollen-stigma interactions, as well as protection against biotic and abiotic stresses. Quantitative comparison of the triticale stigma transcriptome and proteome showed poor correlation, highlighting the importance of having both types of analysis. This work makes a significant contribution towards the elucidation of the Triticeae stigma proteome and provides novel insights into its role in stigma development and function.

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