Presence, viral load and characterization of Torque teno sus viruses in liver and pork chop samples at retail
Leblanc, D., Houde, A., Gagné, M.J., Plante, D., Bellon-Gagnon, P., Jones, T.H., Muehlhauser, V., Wilhelm, B., Avery, B., Janecko, N., Brassard, J. (2014). Presence, viral load and characterization of Torque teno sus viruses in liver and pork chop samples at retail, 178 60-64. http://dx.doi.org/10.1016/j.ijfoodmicro.2014.03.005
Torque teno viruses (TTV) are widespread in humans, swine as well as in several other animal species. In market ready swine, the reported prevalence ranges between 11% and 100%. Through a national retail sampling plan from the Canadian Integrated Program for Antimicrobial Resistance Surveillance (CIPARS) program, 283 and 599 liver and pork chop samples, respectively, were collected over a 12-month period from commercial establishments in 5 selected geographical regions of Canada to assess the presence of Torque teno sus viruses (TTSuVs) in these products.TTSuVs were detected in 97.9% of pork chops with viral loads ranging between 1×104 and 9.9×105 genomic copies (gc)/g and 98.6% of liver samples with viral loads ranging from 1×105 to 9.9×106 gc/g.A selection of 20 positive samples (10 pork chop and 10 liver) from the 5 geographical regions were further tested for the production, of a 305. bp fragment for TTSuV1 and a 253. bp fragment for TTSuV2 in the non-coding region. TTSuV1 was present in all 10 liver and 10 pork chops samples while TTSuV2 was detected in 10 liver and 9 pork chop samples. Two different TTSuV1 sequences were simultaneously detected from 5 of 20 samples and 2 different TTSuV2 sequences were detected from 6 of 19 samples. The omnipresence of TTSuVs in commercial pork samples may allow its use as a viral indicator to monitor the effectiveness of cleaning and disinfecting process in slaughtering, cutting, slicing and packaging facilities. © 2014.
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