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High-throughput species identification of enterococci using pyrosequencing

Zaheer, R., Yanke, L.J., Church, D., Topp, E., Read, R.R., McAllister, T.A. (2012). High-throughput species identification of enterococci using pyrosequencing, 89(3), 174-178. http://dx.doi.org/10.1016/j.mimet.2012.03.012

Abstract

Here we report the development and validation of an automated high-throughput pyrosequencing-based method for the reliable identification of isolated Enterococcus species. This method exploits the discrete species-specificity of hypervariable groES- EL spacer region and utilizes a universal dispensation order optimized for a wide range of Enterococcus species. © 2012.

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