Structural characterization of a highly branched polysaccharide from the seeds of Plantago asiatica L.
Yin, J., Lin, H., Li, J., Wang, Y., Cui, S.W., Nie, S., Xie, M. (2012). Structural characterization of a highly branched polysaccharide from the seeds of Plantago asiatica L., 87(4), 2416-2424. http://dx.doi.org/10.1016/j.carbpol.2011.11.009
In this paper, polysaccharides were extracted from the seeds of Plantago asiatica L. with hot water and separated into three fractions PLP-1 (18.9%), PLP-2 (52.6%) and PLP-3 (28.5%) by SephacrylTM S-400 HR column chomatography. The main fraction PLP-2's structure was elucidated using oxalic acid hydrolysis, partial acid hydrolysis, methylation, GC, GC-MS, 1D and 2D NMR. PLP-2 was composed of Rha, Ara, Xyl, Man, Glc and Gal, in a molar ratio of 0.05:1.00:1.90:0.05:0.06:0.10. Its uronic acid was GlcA. PLP- 2 was highly branched heteroxylan which consisted of a β-1,4-linked Xylp backbone with side chains attached to O-2 or O-3. The side chains consisted of β-T-linked Xylp, α-T-linked Araf, α-T-linked GlcAp, β-Xylp-(1 → 3)-α-Araf and α-Araf-(1 ? 3)-β-Xylp, etc. Based on these results, the structure of PLP-2 was proposed. © 2011 Elsevier Ltd.
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