Release of angiotensin I-converting enzyme inhibitory peptides from flaxseed (linum usitatissimum L.) protein under simulated gastrointestinal digestion
Marambe, H.K., Shand, P.J., Wanasundara, J.P.D. (2011). Release of angiotensin I-converting enzyme inhibitory peptides from flaxseed (linum usitatissimum L.) protein under simulated gastrointestinal digestion, 59(17), 9596-9604. http://dx.doi.org/10.1021/jf202000e
The scope of this study was to determine the ability of flaxseed (Linum usitatissimum L.) proteins to release angiotensin I-converting enzyme inhibitory (ACEI) peptides during simulated gastrointestinal (GI) digestion using a static (SM; no absorption in the intestinal phase) and a dynamic model (DM; simultaneous absorption of digested products in the intestinal phase via passive diffusion). Gastric and gastric + small intestinal digests of flaxseed proteins of both models possessed ACEI activity. The ACEI activity of the gastric + small intestinal digest in the DM (IC 50 unabsorbed, 0.05 mg N/mL; IC 50 absorbed, 0.04 mg N/mL) was significantly higher (p < 0.05) than that of the SM (IC 50, 0.39 mg N/mL). Two peptides, a pentapeptide (Trp-Asn-Ile/Leu-Asn-Ala) and a hexapeptide (Asn-Ile/Leu-Asp-Thr- Asp-Ile/Leu), were identified in the most active ACEI fraction (0.5-1 kDa) of the absorbable flaxseed protein digest by de novo sequencing. © 2011 American Chemical Society.
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