Effect of nitrate and glucose addition on denitrification and nitric oxide reductase (cnorB) gene abundance and mRNA levels in Pseudomonas mandelii inoculated into anoxic soil
Shannon, K.E.M., Saleh-Lakha, S., Burton, D.L., Zebarth, B.J., Goyer, C., Trevors, J.T. (2011). Effect of nitrate and glucose addition on denitrification and nitric oxide reductase (cnorB) gene abundance and mRNA levels in Pseudomonas mandelii inoculated into anoxic soil, 100(2), 183-195. http://dx.doi.org/10.1007/s10482-011-9577-y
The effect of glucose addition (0 and 500 μg C g-1 soil) and nitrate (NO3) addition (0, 10, 50 and 500 μg NO3-N g-1 soil) on nitric oxide reductase (cnorB) gene abundance and mRNA levels, and cumulative denitrification were quantified over 48 h in anoxic soils inoculated with Pseudomonas mandelii. Addition of glucose-C significantly increased cnorB p (P. mandelii and related species) mRNA levels and abundance compared with soil with no glucose added, averaged over time and NO3 addition treatments. Without glucose addition, cnorB p mRNA levels were higher when 500 μg NO3-N g-1 soil was added compared with other NO3 additions. In treatments with glucose added, addition of 50 μg NO3-N g-1 soil resulted in higher cnorB p mRNA levels than soil without NO3 but was not different from the 10 and 500 μg NO3-N g-1 treatments. cnorB p abundance in soils without glucose addition was significantly higher in soils with 500 μg NO3-N g-1 soil compared to lower N-treated soils. Conversely, addition of 500 μg NO3-N g-1 soil resulted in lower cnorB p abundance compared with soil without N-addition. Over 48 h, cumulative denitrification in soils with 500 μg glucose-C g-1 soil, and 50 or 500 μg NO3-N g-1 was higher than all other treatments. There was a positive correlation between cnorB p abundance and cumulative denitrification, but only in soils without glucose addition. Glucose-treated soils generally had higher cnorB p abundance and mRNA levels than soils without glucose added, however response of cnorB p abundance and mRNA levels to NO3 supply depended on carbon availability. © 2011 Springer Science+Business Media B.V.
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