Insecticidal and bactericidal characteristics of the bio-oil from the fast pyrolysis of coffee grounds
Bedmutha, R., Booker, C.J., Ferrante, L., Briens, C., Berruti, F., Yeung, K.K.C., Scott, I., Conn, K. (2011). Insecticidal and bactericidal characteristics of the bio-oil from the fast pyrolysis of coffee grounds, 90(2), 224-231. http://dx.doi.org/10.1016/j.jaap.2010.12.011
The aim of this study was to generate and characterize pyrolysis bio-oil from dried coffee grounds for the purpose of testing its pesticide characteristics towards selected microorganisms and the Colorado Potato Beetle. Dried coffee grounds were pyrolyzed to produce bio-oil. Pyrolysis was carried out at five different temperatures from 400 °C to 600 °C and at a vapor residence time of 5 s, to study the effect of temperature on the yield of the pyrolysis products. The trends clearly indicated a strong effect of temperature on the bio-oil yield. The highest bio-oil yield was found to be 43.8% at 500 °C. Gases were analyzed using gas chromatography-mass spectrometry (GC-MS). Gas yield increased from 17.6% at 400 °C to 32.1% at 600 °C. Char yield decreased from 35.2% at 400 °C to 19.6% at 600 °C. Each of the bio-oils produced between 400 and 600 °C were tested for bactericidal and insecticidal activities against pests found on plants in Canada that currently require improved control options. The dried coffee grounds bio-oil showed significant activity towards two bacteria (Streptomyces scabies and Clavibacter michiganensis subsp. michiganensis) and the Colorado Potato Beetle (Leptinotarsa decemlineata Say). The bio-oil was fractionated and tested for bactericidal and insecticidal activity. The most active fractions were analyzed using GC-MS. While some compounds in the bio-oil were active against both the insect and bacteria, the coffee grounds bio-oil also contained chemicals that provided additional insecticidal activity with no bactericidal activity. © 2011 Elsevier B.V. All rights reserved.
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