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Intestinal mucosa adherence and Cytotoxicity of a Sorbitol-fermenting, shiga-toxin-negative escherichia coli O157:NM isolate with an atypical type III secretion system

Lefebvre, B., Diarra, M.S., Fairbrother, J.M., Nadeau, E., Dubois, M., Malouin, F. (2010). Intestinal mucosa adherence and Cytotoxicity of a Sorbitol-fermenting, shiga-toxin-negative escherichia coli O157:NM isolate with an atypical type III secretion system, 7(8), 985-990. http://dx.doi.org/10.1089/fpd.2009.0474

Abstract

Reports show that sorbitol-fermenting (SF) Escherichia coli O157 isolates are implicated in animal and human diseases and may represent new emerging pathogens. We investigated the cytotoxicity and interaction with intestinal tissues of an SF, Shiga-toxin-negative E. coli O157:NM isolate. This bovine isolate was negative for stx genes and was not cytotoxic for Vero cells. We found that this E. coli O157 isolate possesses an intimin of type β, whereas the translocated intimin receptor Tir and type III secretion system components EspA, EspB, and EspD were of type α. In contrast, Shiga-toxin-positive O157:H7 isolates usually possess variants of type γ. The isolate did not present typical O157:H7 attaching and effacing lesions in the newborn pig ileal in vitro organ culture model. However, extensive effacement and elongation of the microvilli were observed. In vitro organ culture results suggest that such an SF, Shiga-toxin-negative O157:NM isolate found in cattle may potentially cause disease, such as diarrhea without hemolytic uremic syndrome, in humans. © 2010, Mary Ann Liebert, Inc.

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