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Distinct regions at the N-terminus of the Cucumber necrosis virus coat protein target chloroplasts and mitochondria

Hui, E., Xiang, Y., Rochon, D. (2010). Distinct regions at the N-terminus of the Cucumber necrosis virus coat protein target chloroplasts and mitochondria, 153(1), 8-19. http://dx.doi.org/10.1016/j.virusres.2010.06.021

Abstract

Cucumber necrosis virus (CNV) is a spherical virus consisting of 180 identical coat protein (CP) subunits. The N-terminus of the CP subunit contains a 58. aa RNA binding (R) domain and a 34. aa arm that connects the R domain to the shell. These regions are known to play critical roles in virus assembly and disassembly. It has recently been shown that a region encompassing the arm can function as a chloroplast transit peptide (TP) in infected plants and that targeting may represent a means for virus particle disassembly. In this study, we further delineate the TP region and show that a 22. aa sequence at the N-terminus of the shell enhances chloroplast targeting. We also demonstrate that R domain specifically co-localizes with mitochondria in agroinfiltrated plants. Deletion analyses show that the N-terminal 39 amino acids of the R domain are sufficient for mitochondrial targeting and that this region contains features typical of mitochondrial presequences. The R/. arm region is found to be dually targeted to mitochondria and chloroplasts suggesting that this region of the CP plays a critical role in determining the fate of CP during the infection process. © 2010.

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