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A rapid non-destructive DNA extraction method for insects and other arthropods

Castalanelli, M.A., Severtson, D.L., Brumley, C.J., Szito, A., Foottit, R.G., Grimm, M., Munyard, K., Groth, D.M. (2010). A rapid non-destructive DNA extraction method for insects and other arthropods, 13(3), 243-248. http://dx.doi.org/10.1016/j.aspen.2010.04.003

Abstract

Preparation of arthropods for morphological identification often damages or destroys DNA within the specimen. Conversely, DNA extraction methods often destroy the external physical characteristics essential for morphological identification. We have developed a rapid, simple and non-destructive DNA extraction technique for arthropod specimens. This technique was tested on four arthropod orders, using specimens that were fresh, preserved by air drying, stored in ethanol, or collected with sticky or propylene glycol traps. The technique could be completed in 20. min for Coleoptera, Diptera and Hemiptera, and 2. min for the subclass Acarina, without significant distortion, discolouration, or other damage to the specimens. © 2010 Korean Society of Applied Entomology, Taiwan Entomological Society and Malaysian Plant Protection Society.

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