Anaerobic digestion of specified risk materials with cattle manure for biogas production
Gilroyed, B.H., Reuter, T., Chu, A., Hao, X., Xu, W., McAllister, T.A. (2010). Anaerobic digestion of specified risk materials with cattle manure for biogas production, 101(15), 5780-5785. http://dx.doi.org/10.1016/j.biortech.2010.02.077
Biogas production from anaerobic digestion (AD) of specified risk materials (SRM) co-digested with cattle manure was assessed in a 3 × 2 factorial design. SRM replaced manure at 0 (control), 10% or 25% (w/w) as the substrate fed to six 2-L biodigesters maintained at 37 °C or 55 °C. Digesters were fed substrate (30 g L-1 total volatile solids) at 6-d intervals for 90 d, with a retention time of 30 d. Keratin (<20 mg) was added to each digester to model the degradation of β-sheet rich proteins. Methane production was measured daily, and effluent was collected at feeding to monitor SRM degradation using real-time PCR analysis of bovine-specific DNA fragments. Compared with control, methane production increased by 83% or 161% (P < 0.05) with 10% or 25% SRM at 37 °C, and by 45% and 87%, respectively, at 55 °C (P < 0.05). Bovine DNA degradation over 6 d was higher (P < 0.05) at 37 °C as compared to 55 °C. Dry matter degradation of keratin at 37 °C decreased with increasing SRM concentration (P < 0.05), whereas at 55 °C no difference between treatments was observed (P > 0.05). Inclusion of SRM increases the production of methane during the anaerobic digestion of manure and may offer a means of deriving economic value from the disposal of SRM. Crown Copyright © 2010.
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