Field Observations on Tetraploid Red Raspberry Genotypes.
Jamieson, A.R. and McLean, N.L. (2009). "Field Observations on Tetraploid Red Raspberry Genotypes.", Acta Horticulturae (ISHS), 777, pp. 183-187.
Seeds derived from crosses of diploid (2n=2x=14) raspberry genotypes were surface sterilized, cut in half, and placed on tissue culture medium containing colchicine, where they germinated. Surviving plantlets were subsequently rooted in vitro and rooted plantlets were acclimatized to greenhouse conditions. The resulting 146 plants were planted in the field for evaluation of morphology and horticultural characteristics. For a portion of the plants, chromosome numbers were determined by counts in mitotic root tip cells or in meiotic pollen mother cells. It was determined that the number of chloroplasts in leaf guard cells was highly correlated with ploidy; a mean of 10.8 for diploids and 23.1 for tetraploids (2n=4x=28). Preliminary measurements on field-grown plants indicated that ploidy level influences flower size, flower fertility, drupelet numbers and size, seed size, and leaf morphology.
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