Pyramiding multiple genes for resistance to soybean mosaic virus in soybean using molecular markers
Shi, A., Chen, P., Li, D., Zheng, C., Zhang, B., Hou, A. (2009). Pyramiding multiple genes for resistance to soybean mosaic virus in soybean using molecular markers, 23(1), 113-124. http://dx.doi.org/10.1007/s11032-008-9219-x
Seven strains of Soybean mosaic virus (SMV) and three independent resistance loci (Rsv1, Rsv3, and Rsv4) have been identified in soybean. The objective of this research was to pyramid Rsv1, Rsv3, and Rsv4 for SMV resistance using molecular markers. J05 carrying Rsv1 and Rsv3 and V94-5152 carrying Rsv4 were used as the donor parents for gene pyramiding. A series of F2:3, F3:4, and F4:5 lines derived from J05 × V94-5152 were developed for selecting individuals carrying all three genes. Eight PCR-based markers linked to the three SMV resistance genes were used for marker-assisted selection. Two SSR markers (Sat_154 and Satt510) and one gene-specific marker (Rsv1-f/r) were used for selecting plants containing Rsv1; Satt560 and Satt063 for Rsv3; and Satt266, AI856415, and AI856415-g for Rsv4. Five F4:5 lines were homozygous for all eight marker alleles and presumably carry all three SMV resistance genes that would potentially provide multiple and durable resistance to SMV. © 2008 Springer Science+Business Media B.V.
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