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Buchwaldt, Lone, Ph.D.

Research Scientist - Plant Pathology

Contact Information:

107 Science Place
Saskatoon, Saskatchewan S7N 0X2
Telephone: 306-385-9417
Fax: 306-385-9482
Email: lone.buchwaldt@agr.gc.ca

Areas of Expertise:

  • evaluation of plant germplasm for disease resistance
  • genetics of disease resistance in legume crops
  • sclerotinia resistance
  • pathogen variability
  • Evaluation of plant germplasm held at Plant Gene Resources of Canada (PGRC) for resistance to important fungal diseases for utilization of resistant lines in plant breeding; emaphasis on understanding the genetics of resistance to ascochyta blight in chickpea (Cicer aritinum) cause by (Ascochyta rabiei), anthracnose of lentil (Lens culinaris) caused by (Colletotrichum truncatum), and sclerotinia stem rot of canola (Brassica napus) caused by Sclerotinia sclerotiorum; development of molecular markers linked to genes conferring disease resistance to be utilized by plant breeders in marker-assisted-selection of disease resistant cultivars.

Current Projects:

  • Develop new information through evaluation, characterization and analysis of genetic resource attributes
  • Evaluation of canola (Brassica napus) germplasm and Canadian canola cultivars for resistance to stem rot caused by Sclerotinia sclerotiorum for identification of new lines useful in canola breeding programs.
  • Development of microsatellite markers in canola (Brassica napus) linked to quantitative trait loci conferring resistance to stem rot caused by Sclerotinina scleortiorum for utilization by plant breeders in marker-assisted selection of lines with improved stem rot resistance.
  • Development of a molecular linkage map in chickpea based on single nucleotide polymorphism (SNP) with special emphasis on identification of markers closely linked to genes conferring resistance to ascochyta blight caused by Ascochyta rabiei.
  • Elucidation on the genetic basis of resistane to ascohtya blight in chickpea including the genetics of virulence in the pahtogen population in collaboration with plant pathologists and plant breeders nationally and internationally.

Affiliations:

  • Canadian Phytopahtological Soceity 1995 to present; Excecutive Board 1997-2000.
  • The Prairie Registration Recommending Committee on Grain (PRRCG), 1996 to present.
  • Plant Disease Sub-Council of Saskatchewan, 1999 to present.
  • International Sclerotinia Working Group.

Research publications

2016

2015

2013

2010

2009

Other science related publications

2015

  • Blahut-Beatty, L., Koziol, L., Calla, B., Buchwaldt, L., Neece, D.J., Clough, S.J., and Simmonds, D.H. (2015). "Strategies to identify functionally significant defense genes against Sclerotinia sclerotiorum.", USDA-ARS National Sclerotinia Initiative. 12th Annual Meeting, Bloomington, MN, USA, January 22-24, 2014, pp. 32.
  • Blahut-Beatty, L., Koziol, L., Calla, B., Buchwaldt, L., Neece, D.J., Cough, S.J., and Simmonds, D.H. (2015). "Strategies to identify functionally significant defense genes against Sclerotinia sclerotiorum.", USDA-ARS National Sclerotinia Initiative. 13th Annual Meeting, Bloomington, MN, USA, January 21-23, 2015, Number 9, pp. 30. (Abstract)
  • Durkin, J.M.H., Bissett, J.D., Song, J.-R., Pahlavani, M., Mooney, B.G., and Buchwaldt, L. (2015). "Minisatellites in IGS useful for race differentiation in Colletotrichum of lentil and a likely site of small RNA synthesis affecting pathogenicity.", 28th Fungal Genetics Conference, Pacific Grove, CA, USA, March 17-22, 2015, Abstract No. 578; Selected oral presentation, pp. 293. (Abstract)
  • Fu, F., Rimmer, S.R., Lydiate, D.J., Hegedus, D.D., Parkin, I.A.P., Adam, J., Harrington, M., McGregor, L., and Buchwaldt, L. (2015). "Mapping of quantitative trait loci in Brassica napus lines from Pakistan and China providing resistance to the Sclerotinia sclerotiorum.", 14th International Rapeseed Congress, Saskatoon, SK, Canada, July 5-9, 2015. (Abstract)
  • Puri, K.D., Garg, H., Durkin, J.M.H., Adam, J., Harrington, M., Liabeuf, D., Gali, K.K., Sharpe, A.G., and Buchwaldt, L. (2015). "Characterization of the Sclerotinia sclerotiorum population from canola in western Canada needed for selection of partially resistant Brassica napus germplasm.", 14th International Rapeseed Congress, Saskatoon, SK, Canada, July 5-9, 2015. (Abstract)

2014

  • Bissett, J.D. and Buchwaldt, L. (2014). "Sequence repeat patterns in the IGS region distinguish genetically highly similar pathovars of Colletotrichum viciae on lentils.", 10th International Mycological Conference (IMC10), Bangkok, Thailand, August 3-8, 2014.
  • Buchwaldt, L., Hegedus, D.D., Parkin, I.A.P., Vail, S.L., Fu, F., Garg, H., Gyawali, S., and Puri, K.D. (2014). "Dissecting quantitative resistance to sclerotinia in Brassica napus.", Applying Genomics to Canola Improvement Workshop, Saskatoon, SK, Canada, December 4, 2014, Invited oral presentation. (Abstract)
  • Durkin, J.M.H., Bissett, J.D., Song, R., Pahlavani, H., Mooney, B.G., and Buchwaldt, L. (2014). "A change in the Colletotrichum truncatum population signals a need for new resistance in lentil.", 6th International Food Legumes Research Conference, Saskatoon, SK, Canada, July 7-11, 2014, Number 166, pp. 168. (Abstract)
  • Prabhakaran, R., Varshney, R.K., Gali, K.K., Sharpe, A.G., and Buchwaldt, L. (2014). "Resistance in chickpea to Ascochyta rabiei is conferred by genes encoding pathogen recognition and other genes in basic defense pathways.", 6th International Food Legumes Research Conference, Saskatoon, SK, Canada, July 7-11, 2014, Number 20, pp. 94. (Abstract)
  • Puri, K.D., Garg, H., Durkin, J.M.H., Hegedus, D.D., Harrington, M., Adam, J., Liabeuf, D., Gali, K.K., Sharpe, A.G., and Buchwaldt, L. (2014). "Genetic and pathogenic variability of Sclerotinia sclerotiorum from canola in Western Canada.", CPS Regional Meeting, Saskatchewan. SK, Canada. (Abstract)
  • Simmonds, D.H., Calla, B., Blahut-Beatty, L., Koziol, L., Buchwaldt, L., Neece, D.J., and Clough, S.J. (2014). "Gene expression studies to identify and verify the function of soybean defense genes against Sclerotinia sclerotiorum.", 15th Biennial Conference Molecular and Cellular Biology of the Soybean, Minneapolis, Minnesota, USA, August 3-6, 2014, pp. 44.
  • Simmonds, D.H., Calla, B., Blahut-Beatty, L., Koziol, L., Buchwaldt, L., Neece, D.J., and Clough, S.J. (2014). "Gene expression studies to identify and verify the function of soybean defense genes against Sclerotinia sclerotiorum.", 2nd Plant Genomics Congress, Europe, London, UK, May 11-14, 2014. (Poster)
  • Turkington, T.K., Buchwaldt, L., Orchard, D., Strelkov, S.E., Ziesman, B.R., and Lange, R. (2014). "Assessing risk and spray timing for sclerotinia stem rot.", Daily interactive workshops, 2014 CanoLAB training event, Bolting and Beyond Session, Olds, Alberta, Canada, February 19-21, 2014. (Presentation)

2013

  • Buchwaldt, L. (2013). "Dissecting quantitative resistance to Sclerotinia sclerotiorum in Asian Brassica napus germplasm.", Bayer Crop Science, Saskatoon, SK, Canada, February 26, 2013. (Presentation)
  • Buchwaldt, L. (2013). "Dissecting quantitative resistance to Sclerotinia sclerotiorum in Asian Brassica napus germplasm.", Cargill, Aberdeen, SK, Canada, March 14, 2013. (Presentation)
  • Buchwaldt, L. (2013). "Dissecting quantitative resistance to Sclerotinia sclerotiorum in Asian Brassica napus germplasm.", Dow Agro Science, Saskatoon, SK, Canada, January 28, 2013. (Presentation)
  • Buchwaldt, L. (2013). "Dissecting quantitative resistance to Sclerotinia sclerotiorum in Asian Brassica napus germplasm.", National Sclerotinia Initiative 9th Annual Meeting, Minneapolis, MN, USA, January 23-25, 2013. (Presentation)
  • Buchwaldt, L. (2013). "Dissecting quantitative resistance to Sclerotinia sclerotiorum in Asian Brassica napus germplasm.", Viterra – Lantmannen (Svalof-Weibull), Saskatoon, SK, Canada, March 20, 2013. (Presentation)
  • Buchwaldt, L., Durkin, J.M.H., and Garg, H. (2013). "Characterization of S. sclerotiorum clones in western Canada for pathogenicity and genomic relatedness.", WCC/RRC Pathology Subcommittee, Saskatoon, SK, Canada, February 4, 2013.
  • Buchwaldt, L., Fu, F., Gyawali, S., Hegedus, D.D., and Parkin, I.A.P. (2013). "Towards identification of quantitative loci for sclerotinia resistance in Brassica napus germplasm and the underlying defense genes.", GCIRC Technical Meeting, Geneva, Switzerland, April 28- May 1, 2013. (Abstract)
  • Buchwaldt, L., Hegedus, D.D., Lydiate, D.J., Parkin, I.A.P., Fu, F., Garg, H., Gyawali, S., and Rimmer, S.R. (2013). "Dissecting quantitative resistance to Sclerotinia sclerotiorum in Asian Brassica napus germplasm.", National Sclerotinia Initiative 9th Annual Meeting, Minneapolis, MN, USA, January 23-25, 2013.

2012

  • Buchwaldt, L., Hegedus, D.D., and Parkin, I.A.P. (2012). "A genomics approach to sclerotinia resistance in Brassica napus. SaskCanola 20th Anniversary Research Summary."
  • Buchwaldt, L., Hegedus, D.D., and Parkin, I.A.P. (2012). "Characterization of S. sclerotiorum clones in western Canada for pathogenicity and genomic relatedness.", Canola Cluster update, Saskatoon, SK, Canada, March 21, 2012.
  • Buchwaldt, L., Kishore, G.K., Prabhakaran, R., Fu, F., Booker, H.M., Madrid, E.H., Millán, T., Gil, J., Rubin, J., and Sharpe, A.G. (2012). "Status and prospects of controlling ascochyta blight in chickpea by genetic resistance.", 9th Canadian Pulse Crop Research Workshop, Niagara Falls, ON, Canada, November 6-9, 2012. (Abstract)
  • Buchwaldt, L., Lydiate, D.J., Yu, F.Q., Fu, F., Hegedus, D.D., Gyawali, S., Garg, H., and Rimmer, S.R. (2012). "Advances in sclerotinia resistance research.", Canola Industry Meeting, Garden Inn Hotel, Saskatoon, SK, Canada, December 5, 2012. (Presentation)
  • Buchwaldt, L., Shaikh, R., Diederichsen, A., Harrington, M., Adam, J., and Conner, R.L. (2012). "Sources of resistance to Colletotrichum truncatum in lentil obtained by cycles of single plant selection in diverse germplasm.", 9th Canadian Pulse Crop Research Workshop, Niagara Falls, ON, Canada, November 6-9, 2012. (Abstract)
  • Hegedus, D.D., Buchwaldt, L., and Parkin, I.A.P. (2012). "Development of sclerotinia resistant Brassica napus lines and molecular markers for marker-assisted breeding.", Canola Cluster update, Saskatoon, SK, Canada, March 21, 2012.
  • Simmonds, D.H., Blahut-Beatty, L., Koziol, L., Buchwaldt, L., Zhang, Y., Calla, B., Neece, D.J., and Clough, S.J. (2012). "Identification of candidate defence-related genes to Sclerotinia sclerotiorum in soybean; functional analysis in soybean, Arabidopsis, and Nicotiana benthamiana.", 14th Cellular and Molecular Biology of the Soybean Conference, Des Moines, Iowa, USA, August 12-15, 2012, Number 104. (Abstract)
  • Simmonds, D.H., Blahut-Beatty, L., Koziol, L., Buchwaldt, L., Zhang, Y., Calla, B., Neece, D.J., and Clough, S.J. (2012). "Identification of candidate defence-related genes to Sclerotinia sclerotiorum in soybean; functional analysis in soybean, Arabidopsis, and Nicotiana benthamiana.", 6th International Conference on Legume Genetics and Genomics, Hyderabad, India, October 2-7, 2012, Number P-PAD30. (Abstract)
  • Turkington, T.K., Kutcher, H.R., Jurke, C., Buchwaldt, L., and McLaren, D.L. (2012). "Managing sclerotinia stem rot of canola.", Webinar, Canola Council of Canada and canola grower’s associations (ACPC/SC/MCGA), Canada, June 28, 2012. (Presentation)

2011

  • Buchwaldt, L. (2011 Internal). Identification of DNA markers linked to QTLs for Sclerotinia sclerotiorum resistance in spring and winter types of Brassica napus. Final report and oral presentation to Bayer CropScience, Dow AgroScoence, KWS and DL Seeds. (Report)
  • Buchwaldt, L. (2011). "Utilization of sclerotinia resistance in canola from Asia.", Crop Protection Field Tour, Saskatoon Research Farm, Saskatoon, SK, Canada, July 20, 2011. (Presentation)
  • Buchwaldt, L., Hegedus, D.D., and Parkin, I.A.P. (2011). "Sclerotinia resistance in B. napus – an update.", Cargill, Aberdeen, SK, Canada, November 23, 2011. (Presentation)
  • Buchwaldt, L., Lydiate, D.J., Yu, F.Q., Hegedus, D.D., and Rimmer, S.R. (2011). "QTLs contributing to sclerotinia stem rot resistance in Brassica napus Zhongyou 821.", 13th International Rapeseed Congress, Prague, Czech Republic, June 5-9, 2011.
  • Fu, F., Harrington, M., Hegedus, D.D., Bekkaoui, D.R., Parkin, I.A.P., and Buchwaldt, L. (2011). "Co-location of defence genes from expression studies with QTLs conferring sclerotinia resitance in Brassica napus.", 8th Canadian Plant Genomics Workshop, Niagara Falls, ON, Canada, August 22-25, 2011. (Workshop)
  • Gyawali, S., Buchwaldt, L., Harrington, M., Parkin, I.A.P., Bekkaoui, D.R., Horner, K., Higgins, E.E., and Hegedus, D.D. (2011). "Association mapping of stem rot resistance in a world collection of Brassica napus.", American Phytopathological Society (APS) 2011 Joint Annual Meeting & International Association for the Plant Protection Sciences (IPPC), Honolulu, HI, USA, August 6-10, 2011.
  • Hegedus, D.D., Buchwaldt, L., and Parkin, I.A.P. (2011). A genomics approach to sclerotinia resistance in Brassica napus. SaskCanola 20th Anniversary Research Summary., Agriculture and Agri-Food Canada (AAFC)/Agriculture et Agroalimentaire Canada (AAC). (Report)
  • Kutcher, H.R., Dokken-Bouchard, F.L., Turkington, T.K., Fernando, W.G.D., Boyetchko, S.M., Buchwaldt, L., Hegedus, D.D., and Parkin, I.A.P. (2011). "Managing sclerotinia stem rot in canola.", Canola Research Summit meeting, Winnipeg, MB, Canada, April 12-13, 2011.

2010

  • Buchwaldt, L. (2010). "Identification of sclerotinia resistant B. napus and development of molecular markers linked to stem rot resistance.", Canola Industry Meeting 2010, Garden Inn Hotel, Saskatoon, SK, Canada, December 8, 2010, Invited oral presentation. (Abstract)
  • Buchwaldt, L. (2010). "Promising new sources of sclerotinia resistance in Brassica napus from Asia.", Canola Days 2010, Saskatchewan Canola Growers Association, Saskatoon, SK, Canada, January 13, 2010.
  • Buchwaldt, L., Gali, K.K., Sharpe, A.G., Booker, H.M., and Tar’an, B. (2010). "QTL associated with Ascochyta rabiei resistance in chickpea and a suggested labeling system.", 5th International Food Legumes Research Conference (IFLRC V) and 7th European Conference on Grain Legumes (AEP VII), Antalya, Turkey, April 26-30, 2010.
  • Buchwaldt, L., Hegedus, D.D., and Parkin, I.A.P. (2010). "Sclerotinia research at AAFC, Saskatoon.", Canola Industry Meeting, Garden Inn Hotel, Saskatoon, SK, Canada, December 8, 2010. (Presentation)
  • Shaikh, R. and Buchwaldt, L. (2010). "Anthracnose resistance in lentil - genetic inheritance and molecular markers under development.", 8th Canadian Pulse Research Workshop, Calgary, AB, Canada, November 3-5, 2010. (Abstract)

2009

  • Buchwaldt, L., Kishore, G.K., Shaikh, R., Singh, P., Booker, H.M., Tar’an, B., and Sharpe, A.G. (2009). "Utilization of disease resistance genes in chickpea and lentil and their wild relatives.", North American Pulse Improvement Association (NAPIA) Biannual Meeting 2009, Fort Collins, CO, USA, October 28-30, 2009, pp. 5.
  • Zhao, J., Buchwaldt, L., Rimmer, S.R., Sharpe, A.G., McGregor, L., Bekkaoui, D.R., and Hegedus, D.D. (2009). "Patterns of gene expression in Brassica napus cultivars infected with Sclerotinia sclerotiorum.", 14th International Sclerotinia Workshop, Wilmington, NC, USA, May 31-June 4, 2009. (Workshop)
  • Zhao, J., Buchwaldt, L., Rimmer, S.R., Sharpe, A.G., McGregor, L., Bekkaoui, D.R., and Hegedus, D.D. (2009). "Patterns of gene expression in Brassica napus cultivars infected with Sclerotinia sclerotiorum.", 7th Canadian Plant Genomics Workshop, Sheraton Cavalier Hotel, Saskatoon, SK, Canada, August 24-27, 2009. (Workshop)
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