Marsolais, Frédéric, Ph.D.

Research Scientist, Protein Chemistry


Contact Information:

1391 Sandford St
London, Ontario N5V 4T3
Telephone: 519-457-1470 (311)
Fax: 519-457-3997
Email: frederic.marsolais@agr.gc.ca

Areas of Expertise:

  • Protein chemistry of pulses
  • Plant nitrogen metabolism
  • Amino acid metabolism
  • Protein biochemistry
  • Molecular genetics
  • Enzymology
  • Proteolytic processing

Current Projects:

  • Pulse Science Cluster 2
  • Phaseolus genomics for improved bio-product development
  • Identification and characterization of new genes related to nutritional quality, and development of new platform technologies for dry bean improvement
  • Asparagine metabolism and function in nitrogen signalling
  • Genetic improvement of protein quality in edible beans with adaptation to Manitoba

Affiliations:

  • Member, Ontario Pulse Committee
  • Member, Phytochemical Society of North America
  • Member, Canadian Society of Plant Biologists
  • Member, American Society of Plant Biologists
  • Member, Bean Improvement Cooperative

Research Publications:

The links below lead to abstracts with access to full text.

2014

2013

2012

2011

2010

Other Science Related Publications:

2015

  • Pandurangan, S., Crosby, W.L., Pauls, K.P., and Marsolais, F. (2015). "Genomic analysis of polymorphisms conferring storage protein deficiency in common bean.", ASPB Midwestern Section’s Annual Meeting, Saint-Louis, MO, USA, March 21-22, 2015, Presentation. (Poster)

2014

  • Ajewole, E., Pajak, A., and Marsolais, F. (2014). "K+ dependent and K+ independent asparaginases from common bean (Phaseolus vulgaris): mechanism of activation by K+.", Biology Graduate Research Forum, University of Western Ontario, London, ON, Canada, October 3, 2014, Presentation. (Poster)
  • Dhaubhadel, S., Austin, R.S., Marsolais, F., Mainali, H.R., P-Behnami, S., Pajak, A., and Chen, L. (2014). "Seed coat RNAseq analysis of two Pinto bean cultivars that differ in post-harvest seed coat darkening.", Joint conference of the 6th International Food Legumes Research Conference (IFLRC VI) and 7th International Conference on Legume Genetics and Genomics (ICLGG VII), Saskatoon, SK, Canada, July 6-11, 2014.
  • Hou, A., Marsolais, F., Pajak, A., and Conner, R.L. (2014). "Genetic improvement of protein quality in edible beans with adaptation to Manitoba.", Annual Report of the Bean Improvement Cooperative (BIC), 57(129).
  • Joshi, J., Pandurangan, S., and Marsolais, F. (2014). "Analysis of candidate enzymes for the biosynthesis of S-methylcysteine in seed of common bean (Phaseolus vulgaris).", 6th International Food Legumes Research Conference (IFLRC VI) and 7th International Conference on Legume Genetics and Genomics (ICLGG VII), Saskatoon, SK, Canada, July 14-17, 2014, Presentation. (Poster)
  • Joshi, J., Pandurangan, S., and Marsolais, F. (2014). "Analysis of candidate enzymes for the conversion of cysteine into S-methylcysteine in seed of common bean (Phaseolus vulgaris).", Biology Graduate Research Forum, University of Western Ontario, London, ON, Canada, October 3, 2014, Oral presentation.
  • Kambhampati, S., Pandurangan, S., Pajak, A., Austin, R.S., and Marsolais, F. (2014). "Transcriptional responses to exogenous asparagine in Arabidopsis roots.", Eastern Regional Meeting of the Canadian Society of Plant Biologists, Guelph, ON, Canada, November 28-29, 2014. (Poster)
  • Lin, Y., Pajak, A., Marsolais, F., McCourt, P., and Riggs, C.D. (2014). "Characterization of a cruciferin deficient mutant of Arabidopsis and its utility for overexpression of foreign proteins in plants.", 9th Canadian Plant Biotechnology Conference, Montreal, QC, Canada, May12-15, 2014. (Poster)
  • Ma, Z., Marsolais, F., Igamberdiev, A.U., and Bykova, N.V. (2014). "Metabolism of organic acids in barley seeds during germination.", Plant Biology 2014, Portland, Oregon, USA, July 12-16, 2014. (Poster)
  • Marsolais, F. (2014). "Protein Chemistry of grain legumes.", Science Day, Southern Crop Protection and Food Research Centre, London, ON, Canada, December 9, 2014. (Presentation)
  • Marsolais, F. (2014). "Protein chemistry.", Ontario Pulse Research Subcommittee, London, ON, Canada, February 7, 2014. (Presentation)
  • Nowak, J.J., Tian, L.-N., and Marsolais, F. (2014). "Tolerance to the herbicide imazethapyr (Pursuit) in common bean. Oral presentation.", Eastern Regional Meeting of the Canadian Society of Plant Biologists, Guelph, ON, Canada, November 28-29, 2014.
  • Pandurangan, S., Crosby, W., Pauls, K.P., and Marsolais, F. (2014). "Genomic analysis of polymorphisms conferring storage protein deficiency in common bean.", 6th International Food Legumes Research Conference (IFLRC VI) and 7th International Conference on Legume Genetics and Genomics (ICLGG VII), Saskatoon, SK, Canada, July 14-17, 2014. (Poster)
  • Pandurangan, S., Crosby, W.L., Pauls, K.P., and Marsolais, F. (2014). "Genomic analysis of mutations conferring storage protein deficiency in common bean.", Annual Report of the Bean Improvement Cooperative (BIC), 57(29).
  • Pandurangan, S., Sandercock, M., Hou, A., and Marsolais, F. (2014). "Differential response to sulphur nutrition of two common bean genotypes differing in storage protein composition. Poster presentation.", 9th International Workshop on Sulfur Metabolism in Plants "Molecular Physiology and Ecophysiology of Sulfur", Freiburg, Germany, April 14-17, 2014.
  • Zhang, Q., Sutton, M., Pajak, A., and Marsolais, F. (2014). "Identification and characterization of short chain dehydrogenases/reductases (SDRs) catalyzing the reduction of α-ketosuccinamate to α-hydroxysuccinamate in the asparagine catabolic pathway.", Banff Conference on Plant Metabolism, Banff, AB, Canada, June 26-30, 2014. (Poster)
  • Zou, X., Shi, C., Austin, R.S., Merico, D., Munholland, S., Marsolais, F., Navabi, A., Crosby, W.L., Pauls, K.P., Yu, K., and Cui, Y. (2014). "Genome-wide SNP and InDel discovery and transcriptional profiling of CBB resistance in common bean.", 6th International Food Legumes Research Conference (IFLRC VI) and 7th International Conference on Legume Genetics and Genomics (ICLGG VII), Saskatoon, SK, Canada, July 14-17, 2014, Presentation. (Poster)

2013

  • Hou, A., Marsolais, F., Pajak, A., and Conner, R.L. (2013). "Genetic improvement of protein quality in edible beans with adaptation to Manitoba. Poster presentation.", Bean Improvement Cooperatives, Portland, OR, USA, October 28-30, 2013. (Poster)
  • Pandurangan, S., Crosby, W.L., Pauls, K.P., and Marsolais, F. (2013). "Genomic analysis of mutations conferring storage protein deficiency in common bean (Phaseolus vulgaris).", Canada NGS Symposia, Toronto, ON, Canada, June 14, 2013. (Poster)
  • Pandurangan, S., Crosby, W.L., Pauls, K.P., and Marsolais, F. (2013). "Genomic analysis of mutations conferring storage protein deficiency in common bean (Phaseolus vulgaris).", Canadian Plant Genomics Workshop, Halifax, NF, Canada, August 12-15, 2013. (Poster)
  • Pandurangan, S., Crosby, W.L., Pauls, K.P., and Marsolais, F. (2013). "Genomic analysis of mutations conferring storage protein deficiency in common bean.", Bean Improvement Cooperative Meeting, Portland, OR, USA, October 28-29, 2013.

2012

  • Bejger, M., Imiolczyk, B., Gilski, M., Marsolais, F., Pajak, A., and Jaskolski, M. (2012). "The first crystal structure of potassium-dependent Ntn-hydrolase. Oral presentation.", 15th Heart of Europe Biocrystallography Meeting (HEC-15), Beilngries, Germany, September 28-30, 2012.
  • Dhaubhadel, S. and Marsolais, F. (2012). "Transcriptomics of Legume Seed: Soybean a Model Grain Legume.", in Agrawal, G.K. and Rakwal, R. (eds.) - Seed Development: OMICS Technologies toward Improvement of Seed Quality and Crop Yield. OMICS in Seed Biology, Springer, Chapter 8, pp. 129-142.
  • Hou, A., Marsolais, F., Pajak, A., and Conner, R.L. (2012). "Genetic improvement of protein quality in edible beans with adaptation to Manitoba.", 9th Canadian Pulse Research Workshop, Niagara Falls Marriott Gateway, Niagara Falls, ON, Canada, November 6-12, 2012, Poster.
  • Kilpatrick, K.G., Krizevski, R., Hagel, J.M., Lewinsohn, E., Facchini, P.J., and Marsolais, F. (2012). "Functional genomic investigation of ephedrine alkaloid biosynthesis in Ephedra sinica – cloning and characterization of aminotransferases.", 3rd Annual Biology Graduate Research Forum 2012, University of Western Ontario, London, ON, Canada, October 20, 2012, Oral presentation.
  • Kilpatrick, K.G., Krizevski, R., Hagel, J.M., Lewinsohn, E., Facchini, P.J., and Marsolais, F. (2012). "Functional genomic investigation of ephedrine alkaloid biosynthesis in Ephedra sinica – cloning and characterization of aminotransferases.", 51st Phytochemical Society of North America (PSNA) Annual Meeting, University of Western Ontario, London, ON, Canada, August 11-15, 2012, Published in: Pharm Biol 50: 1345.
  • Kilpatrick, K.G., Krizevski, R., Hagel, J.M., Lewinsohn, E., Facchini, P.J., and Marsolais, F. (2012). "Functional genomic investigation of ephedrine alkaloid biosynthesis in Ephedra sinica – cloning and characterization of aminotransferases.", Pharmaceutical Biology, 50, pp. 1345. (Abstract)
  • Liao, D., Pajak, A., and Marsolais, F. (2012). "Transcripts of sulfur metabolic genes are coordinately regulated by protein demand in seed lacking phaseolin and major lectins.", Phaseomics – The Genome Meeting, Guanajuato, México, June 21-23, 2012. (Presentation)
  • Pandurangan, S. and Marsolais, F. (2012). "Genomic analysis of storage protein deficient lines of common bean.", 9th Canadian Pulse Research Workshop, Niagara Falls Marriott Gateway, Niagara Falls, ON, Canada, November 6-12, 2012. (Poster)
  • Pandurangan, S., Pajak, A., Austin, R.S., and Marsolais, F. (2012). "Transcriptional responses to exogenous asparagine in Arabidopsis roots.", 51st Phytochemical Society of North America (PSNA) Annual Meeting, University of Western Ontario, London, ON, Canada, August 11-15, 2012.
  • Pandurangan, S., Pajak, A., Marsolais, F., and Austin, R.S. (2012). "Transcriptional responses to exogenous asparagine in Arabidopsis roots.", Pharmaceutical Biology, 50, pp. 1341. (Abstract)
  • Perry, G.E., Crosby, W.L., Yu, K., Navabi, A., Zou, X., Power, K.A., Marsolais, F., Xie, W., Weersink, A., Tsao, R., Cooper, D., and Pauls, K.P. (2012). "Sequencing the bean genome: Using comparative genomics to examine interspecific introgressions.", Canadian Society of Plant Biologists Meeting (CSPB 2012), University of Alberta Campus, Edmonton, AB, Canada, June 25-27, 2012. (Presentation)
  • Schernthaner, J.P., Singh, J., Labbé, N.M., Liao, D., and Marsolais, F. (2012). "The unfolded protein response in plant seeds.", in Hendriks, B.P. (ed.) - Agricultural Research Updates. Vol. 2, Nova Science Publishers Inc, pp. 429-454.
  • Zhang, Q., Pajak, A., and Marsolais, F. (2012). "Identification and characterization of AGT1 as an asparagine aminotransferase in Arabidopsis thaliana. Poster presentation.", Canadian Society of Plant Biologists Meeting (CSPB 2012), University of Alberta Campus, Edmonton, AB, Canada, June 25-27, 2012.

2011

  • Kilpatrick, K.G., Krizevski, R., Hagel, J.M., Lewinsohn, E., Facchini, P.J., and Marsolais, F. (2011). "Functional genomics investigation of aminotransferases involved in ephedrine alkaloid biosynthesis in Ephedra sinica Stapf.", 8th Canadian Plant Genomics Workshop, Niagara Falls, ON, Canada, August 22-25, 2011. (Presentation)
  • Kilpatrick, K.G., Krizevski, R., Hagel, J.M., Lewinsohn, E., Facchini, P.J., and Marsolais, F. (2011). "Functional genomics investigation of aromatic aminotransferase enzymes involved in ephedrine alkaloid biosynthesis.", 2nd Annual Biology Graduate Research Forum, University of Western Ontario, London, ON, Canada, October 22, 2011. (Poster)
  • Lee, J. and Marsolais, F. (2011). "Genetic characterization of the response to exogenous asparagine in asparaginase double knock-out mutants of Arabidopsis thaliana seedlings.", Ontario Biology Day 2011, Wilfrid Laurier University, Waterloo, ON, Canada, March 12-13, 2011.
  • Perry, G.E., Crosby, W., Shi, C., Power, K.A., Marsolais, F., Xie, W., Weersink, A., Tsao, R., Cooper, D.M., and Pauls, K.P. (2011). "Sequencing the bean genome: the applied bean genomics and bioproducts project.", Plant Canada 2011, Saint Mary’s University, Halifax, NS, Canada, July 17-21, 2011. (Poster)
  • Perry, G.E., Crosby, W., Yu, K., Shi, C., Powers, K., Marsolais, F., Xie, W., Weersink, A., Tsao, R., Siddiqua, M., Cooper, D.M., and Pauls, K.P. (2011). "The applied bean genomics and bioproducts project: sequencing the bean genome.", Biennial Meeting of the Bean Improvement Cooperative and North American Pulse Improvement Association (BIC/NAPIA 2011), Verdanza Hotel, San Juan, Puerto Rico, October 30-November 2, 2011.
  • Zhang, Q. and Marsolais, F. (2011). "Metabolism and transcriptional responses to asparagine in Arabidopsis thaliana.", 2nd Annual Biology Graduate Research Forum, University of Western Ontario, London, ON, Canada, October 22, 2011. (Poster)

2009

  • Ivanov, A.G. and Marsolais, F. (2009). "Functional characterization and regulation of asparaginases in Arabidopsis. Poster presentation.", 2009 Annual Meeting of the Canadian Society of Plant Physiologists, Simon Fraser University, Burnaby, BC, Canada, June 12-15, 2009.
  • Marsolais, F., Pajak, A., Yin, F., Huang, S.Z., and Chapman, R.A. (2009). "Proteomic Analysis of Seed Storage Protein Deficiency in Common Bean.", Phaseomics VI Meeting, Palace of Salcedo, Pontevedra, Spain, May 21-23, 2009, Actas de la Asociación Española de Leguminosas 4, pp. 53.