Menassa, Rima, Ph.D.

Research Scientist, Plant molecular biology/biotechnology


Contact Information:

1391 Sandford St
London, Ontario N5V 4T3
Telephone: 519-457-1470 (270)
Fax: 519-457-3997
Email: rima.menassa@agr.gc.ca

Areas of Expertise:

  • Production of recombinant proteins in plants, recombinant protein trafficking in the secretory system, recombinant protein accumulation and degradation in vivo.

Current Projects:

  • Analysis of the regulation of transgene expression in plants; inefficient translation or protein degradation?
  • Production of a recombinant protein in tobacco for the treatment of cancer

Affiliations:

  • Adjunct professor, University of Western Ontario, Department of Biology
  • Member, Canadian Society for Plant Physiologists

Research Publications:

The links below lead to abstracts with access to full text.

2013

2012

2011

2010

2009

Other Science Related Publications:

2013

  • Kolotilin, I., Kaldis, A., Pereira, E.O., Laberge, S., and Menassa, R. (2013). "Diverse Effects of Chloroplast Expression Cassette Configuration on Transplastomic Production of Hemicellulases in Tobacco.", 3rd International Symposium on Chloroplast Genomics and Genetic Engineering, Rutgers Univerisity, New Brunswick, NJ, USA, May 8-11, 2013, L33, P603, Published in: Biotechnology for Biofuels. 6(1):65.

2012

  • Chen, L., Kaldis, A., Menassa, R., and Dhaubhadel, S. (2012). "Identification and Characterization of a Cysteine Protease Gene in Tobacco for Use in Recombinant Protein Production.", 51st Phytochemical Society of North America (PSNA) Annual Meeting, University of Western Ontario, London, ON, Canada, August 11-15, 2012.
  • Kolotilin, I., Kaldis, A., Devriendt, B., Joensuu, J.J., Cox, E., and Menassa, R. (2012). "Production of a subunit vaccine candidate against porcine post weaning diarrhea in high biomass transplastomic tobacco.", 8th Canadian Plant Biotechnology Conference, University of Guelph, Guelph, ON, Canada, May 14-17, 2012. (Poster)
  • Menassa, R. (2012). "Protein fusions for high level accumulation and purification of plant-made recombinant proteins.", Queen’s University, Department of Biology, Kingston, ON, Canada, November 9, 2012. (Presentation)
  • Menassa, R., Ahmad, A., and Joensuu, J.J. (2012). "Transient Expression Using Agroinfiltration and Its Applications in Molecular Farming.", in Wang, A. and Ma, S. (eds.) - Molecular Farming in Plants: Recent Advances and Future Prospects, Springer, Chapter 9, pp. 183-198.
  • Pereira, E.O., Kolotilin, I., and Menassa, R. (2012). "Heterologous expression of enzymatic cocktail components for biomass deconstruction.", Plant Cell Walls Gordon Research Conference, Colby College, Waterville, ME, USA, August 4-5, 2012. (Poster)
  • Pereira, E.O., Tsang, A., McAllister, T.A., and Menassa, R. (2012). "The expression and characterization of new active lipase from Acremonium alcalophilum using tobacco plants as bioreactor.", 8th Canadian Plant Biotechnology Conference, University of Guelph, Guelph, ON, Canada, May 14-17, 2012.
  • Saberianfar, R., Gutierrez, S., Joensuu, J.J., Kohalmi, S.E., and Menassa, R. (2012). "Characterizing the critical elements of protein body biogenesis by transient expression of elastin-like polypeptide and hydrophobin fusion tags in N. benthamiana leaves.", 8th Canadian Plant Biotechnology Conference, University of Guelph, Guelph, ON, Canada, May 14-17, 2012.
  • Zhu, H., Brandle, J.E., and Menassa, R. (2012). "Characterization and Evaluation of a Recombinant Hyperthermostable a-Amylase Expressed in Tobacco.", 8th Canadian Plant Biotechnology Conference, University of Guelph, Guelph, ON, Canada, May 14-17, 2012. (Poster)

2011

  • Gutierres, S., Saberianfar, R., and Menassa, R. (2011). "Protein Bodies formation in stable transgenic plants of Nicotiana tabacum expressing elastin-like polypeptide and hydrophobin fusions.", 8th Canadian Plant Genomics Workshop, Niagara Falls, ON, Canada, August 22-25, 2011.
  • Kaldis, A., Brandle, J.E., and Menassa, R. (2011). "BY-2 cells as a production platform for intracellular recombinant proteins.", 8th Canadian Plant Genomics Workshop, Niagara Falls, ON, Canada, August 22-25, 2011.
  • Menassa, R. (2011). "Developing novel bioproducts: plant-made pharmaceuticals and industrial enzymes.", Department of Biology, McGill University, Montréal, QC, Canada, April 4, 2011. (Presentation)
  • Menassa, R. (2011). "Protein fusions for high level accumulation and purification of plant-made recombinant proteins.", Biotechnology Havana 2011, Havana, Cuba, November 28-December 3, 2011.
  • Pereira, E.O., Kolotilin, I., and Menassa, R. (2011). "Production and characterization of polygalacturanase from Aspergillus niger and its fusions with hydrophobin and ELP tag using Nicotiana benthamiana as bioreactor.", 1st Brazilian BioEnergy Science and Technology Conference, Campos do Jordão, São Paulo, SP, Brazil, August 14-18, 2011.
  • Saberianfar, R., Gutierres, S., Kohalmi, S.E., Joensuu, J.J., Conley, A.J., and Menassa, R. (2011). "Protein body induction by elastin-like polypeptide and hydrophobin, a new approach to high-level recombinant protein production.", 8th Canadian Plant Genomics Workshop, Niagara Falls, ON, Canada, August 22-25, 2011.
  • Saberianfar, R., Joensuu, J.J., Conley, A.J., and Menassa, R. (2011). "Elastin-like polypeptide and hydrophobin induced protein bodies share a similar sequestration mechanism from the endoplasmic reticulum, useful for high-level recombinant protein production.", 2nd Annual Biology Graduate Research Forum, University of Western Ontario, London, ON, Canada, October 22, 2011, P19, pp. 17.
  • Zhu, H., Reynolds, B.L., Brandle, J.E., and Menassa, R. (2011). "Expression of a-amylase, a starch degrading enzyme, in tobacco.", 8th Canadian Plant Genomics Workshop, Niagara Falls, ON, Canada, August 22-25, 2011.

2010

  • Kolotilin, I., Pereira, E.O., and Menassa, R. (2010). "Tobacco chloroplast genome engineering - assessment of different integration sites for operon and single gene transformation for high-level expression of cellulolytic enzymes.", Cellulosic Biofuel Network (CBN) 2nd Annual General Meeting, Ottawa Marriott Hotel, Ottawa, ON, Canada, March 11-12, 2010, Poster #104, pp. 54. (Poster)
  • Pereira, E.O., Kolotilin, I., Conley, A.J., and Menassa, R. (2010). "Enhancing production of cell wall degrading enzymes in tobacco.", Cellulosic Biofuel Network (CBN) 2nd Annual General Meeting, Ottawa Marriott Hotel, Ottawa, ON, Canada, March 11-12, 2010, Poster #100, pp. 53. (Poster)