Tambong, James, Ph.D.

Research Scientist


Contact Information:

960 Carling Ave
Ottawa, Ontario K1A 0C6
Telephone: 613-715-5398 (office)
Fax: 613-759-1701
Email: james.tambong@agr.gc.ca

Areas of Expertise:

  • Invasive/quarantine bacterial plant pathogens
  • Genomics of phytobacteria
  • Metagenomics
  • Bacterial taxonomy
  • Molecular detection of phytobacterial pathogens
  • Bacterial biodiversity in soil health and agricultural sustainability
  • Beneficial and pathogenic bacteria of cereals and pulses
  • Biopesticides (Bioresources)

Current Projects:

  • Monitoring an invasive bacterial pathogen (Clavibacter michiganensis subsp. nebraskensis) of corn using next-generation sequencing
  • Mycology and bacteriology biosystematics – Filling gaps in agricultural fungal and bacterial biodiversity
  • Next-generation sequencing – genomics and metagenomics of quarantine fungal and bacterial crop pathogens
  • Development of identification and analysis tools for amplicon-based metagenomics, focusing on high-risk and regulated pathogens

Affiliations:

  • Adjunct Professor, Department of Plant Science, University of Manitoba, MB, Canada
  • Member, Advisory committee, Baccalaureate in Applied Biotechnology, La Cité Collégiale, Ottawa, Ontario, Canada.
  • Section Editor, Bacteria and Phytoplasmas,Canadian Journal of Plant Pathology
  • Member, American Phytopathological Society (Former Chair, Tropical Plant Pathology Committee)
  • Member, American Society for Microbiology (ASM online Minority Mentoring Awardee, 2011)
  • Member, Canadian Phytopathological Society

Research Publications:

The links below lead to abstracts with access to full text.

2015

2014

2013

2012

2011

2010

2009

Other Science Related Publications:

2015

  • Khan, I.U.H., Topp, E., Chen, W., Lapen, D.R., Lévesque, C.A., Pintar, K.D.M., Tambong, J.T., Talbot, G., and Wilkes, G.A. (2015). "Assessment of Zoonotic Pathogens in Agroecosystem.", Biodiversity, Bioresources and Collection Science Day, Ottawa Research and Development Center, Agriculture and Agri-Food Canada, Ottawa, ON, Canada, December 1-2, 2015. (Presentation)
  • Lévesque, C.A., Chen, W., Hambleton, S., Seifert, K.A., Adam, Z., Bilodeau, G.J., Cullis, J., Demeke, T., Dumonceaux, T.J., Gagnon, M., Graefenhan, T., Kawchuk, L.M., Khan, I.U.H., Lewis, C.T., Li, X., Links, M.G., Mimee, B., Rott, M., Tambong, J.T., Topp, E., Yu, Q., and Yuan, K. (2015). Project CRTI 09-462RD - Next generation sequencing, direct detection and genotyping of fungi, bacteria and nematodes in the agri-food system. DRDC-RDDC-2015-C148. Final Close-Out Report. (Report)
  • Lévesque, C.A., Chen, W., Hambleton, S., Seifert, K.A., Adam, Z., Bilodeau, G.J., Cullis, J., Demeke, T., Dumonceaux, T.J., Gagnon, M., Graefenhan, T., Kawchuk, L.M., Khan, I.U.H., Lewis, C.T., Li, X., Links, M.G., Mimee, B., Rott, M., Tambong, J.T., Yu, Q., and Yuan, K. (2015). Project CRTI 09-462RD - Next generation sequencing, direct detection and genotyping of fungi, bacteria and nematodes in the agri-food system. Final Report. 54. http://cradpdf.drdc-rddc.gc.ca/PDFS/unc195/p802330_A1b.pdf. (Report)

2014

  • Khan, I.U.H., Cloutier, M., Topp, E., Lapen, D.R., Lévesque, C.A., Edge, T.A., Tambong, J.T., Talbot, G., and Wilkes, G.A. (2014). "Quantitative Detection and Identification of Arcobacter, Campylobacter and Helicobacter Species in a Canadian Agricultural Watershed.", XVIth International Congress of Virology - International Union of Microbiological Societies (IUMS 2014), July 27 - August 1, 2014, Montréal, Québec, Canada, July 27-August 1, 2014.
  • Li, X., Yuan, K., Nie, J., Adam, Z., Tambong, J.T., Chen, W., Lewis, C.T., De Boer, S.H., and Lévesque, C.A. (2014). "Comparative Genomics Approaches for Detection and Identification of Ralstonia solanacearum race 3 bv 2.", 13th International Conference of Plant Pathogenic Bacteria, Shanghai, China, June 8-13, 2014, Oral presentation. (Abstract)
  • Tambong, J.T., Adam, Z., Lewis, C.T., Lévesque, C.A., Chen, W., Khan, I.U.H., Bromfield, E.S.P., and Xu, R. (2014). "Comparative genomics of Pantoeastewartii subspecies and development of a TaqManReal-Time PCR assay for specific detection of P. stewartii subsp.stewartii.", 13th International Conference of Plant Pathogenic Bacteria, Shanghai, China, June 8-13, 2014, pp. 34. (Abstract)
  • Yuan, K., Li, X., Adam, Z., Tambong, J.T., Lévesque, C.A., Chen, W., Lewis, C.T., and De Boer, S.H. (2014). "Comparative genomics for re-classification of Pectobacterium wasabiae.", 3rd International Workshop on Erwinia, Shanghai, China, June 8-13, 2014, pp. 27-28. (Abstract)
  • Yuan, K., Li, X., Adam, Z., Tambong, J.T., Lévesque, C.A., Chen, W., Lewis, C.T., and De Boer, S.H. (2014). "Draft genome sequence of Pectobacterium wasabiae strain CFIA1002.", 3rd International Workshop on Erwinia, Shanghai, China, June 8-13, 2014.

2013

  • Kaneza, C.A., Whiteduck-Leveillee, K., Whiteduck-Leveillee, J., Laprade, N., Topp, E., Guylaine, T., Lapen, D.R., Tambong, J.T., Lévesque, C.A., and Khan, I.U.H. (2013). "Identification and detection of Arcobacter spp. From water and fecal matter using genus- and species-specific PCR assays.", La Cité Collégiale, Ottawa, ON, Canada, April 26, 2013.
  • Kaneza, C.A., Whiteduck-Leveillee, K., Whiteduck-Leveillee, J., Laprade, N., Topp, E., Guylaine, T., Lapen, D.R., Tambong, J.T., Lévesque, C.A., and Khan, I.U.H. (2013). "Identification and detection of Arcobacter spp. from water and fecal matter using genus- and species-specific PCR assays.", La Cité Collégiale, Ottawa, ON, Canada, April 26, 2013. (Poster)
  • Khan, I.U.H., Cloutier, M., Edge, T.A., Schellhorn, H.E., Gupta, R.S., Laprade, N., Topp, E., Guylaine, T., Lapen, D.R., Tambong, J.T., Wilkes, G.A., Nowak, E.K., and Hill, S. (2013). "A comparative study on the prevalence of thermophilic Campylobacter spp. In agricultural and recreational waters.", Canadian Society of Microbiologists (CSM) 63rd Annual Conference / Conférence annuelle de la Société Canadienne des Microbiologistes (SCM), Ottawa, ON, Canada, June 17-20, 2013.
  • Léveillée, K., Laprade, N., Léveillée, W.J., Topp, E., Lapen, D.R., Tambong, J.T., Talbot, G., Wilkes, G.A., and Khan, I.U.H. (2013). "Antimicrobial resistance profiling of Arcobacter butzleri isolates from faecal sources and an agricultural watershed.", 63rd Annual Conference of the Canadian Society of Microbiologists, Carleton University, Ottawa, ON, Canada, June 17-20, 2013, Abstract AE87.
  • Léveillée, W.J., Kaneza, C.A., Léveillée, K., Talbot, G., Topp, E., Lapen, D.R., Tambong, J.T., Lévesque, C.A., and Khan, I.U.H. (2013). "Optimization of a genus-specific PCR assay and restriction fragment length polymorphism (RFLP) analysis for rapid detection.", 63rd Annual Conference of the Canadian Society of Microbiologists, Carleton University, Ottawa, ON, Canada, June 17-20, 2013, Abstract AE88.
  • Léveillée, W.J., Kaneza, C.A., Léveillée, K., Talbot, G., Topp, E., Lapen, D.R., Tambong, J.T., Lévesque, C.A., and Khan, I.U.H. (2013). "Optimization of multiplex PCR assays for the detection of virulence genes in Arcobacter species isolated from fecal samples.", 63rd Annual Conference of the Canadian Society of Microbiologists, Carleton University, Ottawa, ON, Canada, June 17-20, 2013, Abstract AE86.
  • Laprade, N., Whiteduck-Léveillée, J., Whiteduck-Leveillee, K., Cloutier, M., Tambong, J.T., Topp, E., Lapen, D.R., Wilkes, G.A., and Khan, I.U.H. (2013). "Detection of virulence, toxin and tet(O) resistant genes in thermophilic Campylobacter species using multiplex PCR assays.", Canadian Society of Microbiologists (CSM) 63rd Annual Conference / Conférence annuelle de la Société Canadienne des Microbiologistes (SCM), Ottawa, ON, Canada, June 17-20, 2013.
  • Whiteduck-Leveillee, J., Whiteduck-Leveillee, K., Kaneza, C.A., Cloutier, M., Laprade, N., Topp, E., Guylaine, T., Lapen, D.R., Tambong, J.T., Lévesque, C.A., and Khan, I.U.H. (2013). "Multiplex PCR assay for the detection and identification of virulent genes in Arcobacter species.", Canadian Society of Microbiologists (CSM) 63rd Annual Conference / Conférence annuelle de la Société Canadienne des Microbiologistes (SCM), Ottawa, ON, Canada, June 17-20, 2013.
  • Whiteduck-Leveillee, K., Laprade, N., Whiteduck-Leveillee, J., Topp, E., Kaneza, C.A., Lapen, D.R., Tambong, J.T., Guylaine, T., Wilkes, G.A., and Khan, I.U.H. (2013). "Antimicrobial resistance profiling of Arcobacter butzleri isolated from a Canadian agricultural watershed.", Canadian Society of Microbiologists (CSM) 63rd Annual Conference / Conférence annuelle de la Société Canadienne des Microbiologistes (SCM), Ottawa, ON, Canada, June 17-20, 2013.
  • Whiteduck-Leveillee, K., Whiteduck-Leveillee, J., Kaneza, C.A., Laprade, N., Topp, E., Guylaine, T., Lapen, D.R., Tambong, J.T., Lévesque, C.A., and Khan, I.U.H. (2013). "Development of genus-specific PCR assay for rapid identification of Arcobacter species: Optimization of PCR Primers and Conditions, and Restriction Pattern Analysis.", Canadian Society of Microbiologists (CSM) 63rd Annual Conference / Conférence annuelle de la Société Canadienne des Microbiologistes (SCM), Ottawa, ON, Canada, June 17-20, 2013.

2011

  • Kaneza, C.A., Tambong, J.T., and Xu, R. (2011). "L’Étude de la Phylogénie Moléculaire des souches de Pantoea stewartii.", COOP training, La Cité Collégiale, Ottawa, ON, Canada, September 2011. (Presentation)
  • Tambong, J.T. (2011). "Molecular Phylogeny: Methods and a case study of the genus Pseudomonas.", La Cite Collegiale, Ottawa, ON, Canada, March 22, 2011. (Presentation)
  • Tambong, J.T. and Xu, R. (2011). "Specificity and Sensitivity of a TaqMan real-time PCR assay for detection of several pathovars of Pseudomonas syringae.", Plant Canada 2011, Saint Mary’s University, Halifax, NS, Canada, July 17-21, 2011.

2009

  • Bromfield, E.S.P., Tambong, J.T., Cloutier, S., Prévost, D., Laguerre, G., van Berkum, P., Van Tran Thi, T., Assabgui, R., and Barran, L.R. (2009). "Novel bacterial genotypes isolated from nodules of Medicago sativa and Melilotus alba at a Canadian site without a history of legume cultivation.", 3rd Congress of European Microbiologists (FEMS 2009), Gothenburg Convention Centre, Gothenburg, Sweden, June 28-July 2, 2009, pp. 451. (Abstract)
  • Tambong, J.T., Xu, R., and Bromfield, E.S.P. (2009). "Culture-Independent Analyses of Pseudomonas community structures in soils under Different Agricultural Regimes by DGGE and PCR-based Cloning.", 3rd Congress of European Microbiologists (FEMS 2009), Gothenburg Convention Centre, Gothenburg, Sweden, June 28-July 2, 2009, pp. 553. (Abstract)
  • Zhang, J.X., Xue, A.G., and Tambong, J.T. (2009). "Evaluation of Bacillus subtilis strains for biocontrol of soybean root rots caused by Fusarium oxysporum and F. graminearum.", VIII World Soybean Research Conference, Beijing, China, August 10-15, 2009, pp. 162.
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