Bykova, Natalia, Ph.D.

Research Scientist


Contact Information:

195 Dafoe Rd
Winnipeg, Manitoba R3T 2M9
Telephone: 204-983-1465
Fax: 204-983-4604
Email: bykovan@agr.gc.ca

Areas of Expertise:

  • proteomics
  • biochemistry
  • response to disease and stress
  • mass spectrometry
  • Functional modification-specific proteomics - mass spectrometry-driven identification and characterisation of posttranslational modifications phosphorylation, glycosylation and cysteine oxidorecuction, and elucidation of modification-function relationships
  • Determination of the role of posttranslational protein modifications in coordination of signal transduction pathways induced in plants during biotic, abiotic stress response and seed development
  • Identification of signalling components and the molecular mechanisms of plant-pathogen interactions
  • Glycomic analysis of structures, site specific distribution and composition of N-glycans in transgenic plants with recombinant glycoproteins intended for therapeutic purposes and as potential allergens.

Current Projects:

  • Redox-signalling in germinating wheat seeds using disulfide-proteomics based approach
  • Strategic application of proteomics and plant genotyping toward improved FHB resistance in Canadian wheat.
  • Proteomic approaches for identifying pathogen and host factors eliciting disease or resistance in the wheat-leaf rust interaction

Affiliations:

  • Member of the American Society for Mass Spectrometry

Research Publications:

The links below lead to abstracts with access to full text.

2016

2015

  • An, D., Bykova, N.V., and Debnath, S.C. (2015). "EST-PCR, EST-SSR and ISSR markers to identify a set of wild cranberries and evaluating their relationships.", Canadian Journal of Plant Science, 95(6), pp. 1155-1165. doi : 10.4141/CJPS-2015-158
  • Bykova, N.V., Hu, J.-N., Ma, Z., and Igamberdiev, A.U. (2015). "The role of reactive oxygen and nitrogen species in bioenergetics, metabolism and signaling during seed germination.", in Gupta, K.J. and Igamberdiev, A.U. (eds.) - Reactive Oxygen and Nitrogen Species Signaling and Communication in Plants, Springer Berlin / Heidelberg, pp. 177-195.
  • Hu, J., Rampitsch, C., and Bykova, N.V. (2015). "Advances in plant proteomics toward improvement of crop productivity and stress resistance.", Frontiers in Plant Science, 6(209), pp. 1-15. doi : 10.3389/fpls.2015.00209
  • Igamberdiev, A.U. and Bykova, N.V. (2015). "Non-coupled pathways of plant mitochondrial electron transport and the maintenance of high photorespiratory flux.", in Gupta, K.J., Mur, L.A.J., and Neelwarne, B. (eds.) - Alternative Respiratory Pathways in Higher Plants, John Wiley & Sons, Ltd, Chapter 2.

2014

2013

2012

2011

2010

2009

  • Rampitsch, C. and Bykova, N.V. (2009). "Methods for Functional Proteomic Analyses.", in Somers, Daryl J. et. Al. (eds.) - Plant Genomics: Methods and Protocols (Methods in Molecular Biology), Human Sciences Press, New York, NY, USA.

Other Science Related Publications:

2016

  • Bykova NV, Jordan M, Hu J, Radovanovic N, Rampitsch M (2016) Integrated proteogenomics approach to reveal sources of pre-harvest sprouting resistance in Canadian wheat. 3rd Canadian Wheat Symposium, Ottawa, Ontario, Canada, November 22-25. Poster - P42.
  • Bykova NV, Jordan M, Hu J, Radovanovic N, Rampitsch M (2016) Integration of quantitative proteomics, redox metabolomics and proteogenomics to identify sources of seed dormancy control and pre-harvest sprouting resistance in wheat. 2nd International Plant Proteomics Organization (INPPO) World Congress. Bratislava, Slovakia, September 4th-8th, 2016. Oral presentation
  • Hu J, Radovanovic N, Bykova NV (2016) Integration of proteome and targeted redox metabolome profiling of seed dormancy genotype and after-ripening induced changes in wheat. Plant Biotech 2016, A joint meeting of the CSPB and the Canadian Association for Plant Biotechnology, Kingston, Ontario, Canada, June 19th-21st, 2016. Oral presentation, student.

2015

  • Bykova, N.V. and Igamberdiev, A.U. (2015). "Non-coupled pathways of plant mitochondrial electron transport support high rates of glycine oxidation.", 9th International Conference for Plant Mitochondrial Biology ICPMB, Wrocław, Poland, May 17-22, 2015, P81. (Poster)
  • Bykova, N.V., Hu, J., and Radovanovic, N. (2015). "Redox control of metabolic reprogramming toward seed dormancy release in wheat.", 12th International Conference on Reactive Oxygen and Nitrogen Species in Plants: from model systems to field, Verona, Italy, June 24-26, 2015.
  • Hu, J., Radovanovic, N., Fan, T., and Bykova, N.V. (2015). "Redox regulation of seed dormancy release in spring wheat revealed through tissue-specific proteomics, metabolomics and antioxidant activity profiling.", Canadian Plant Science Annual Meeting with Plant Canada and other plant societies at Botany 2015, Edmonton, Alberta, Canada, July 25-29, 2015. (Poster)

2014

  • Bykova, N.V., Hu, J.-N., Radovanovic, N., You, F.M., and Fan, T. (2014). "Quantitative proteomic approaches to revealing mechanisms of seed dormancy control and pre-harvest sprouting resistance in wheat.", 1st INPPO World Congress on Plant Proteomics: Methodology to Biology, Hamburg, Germany, August 31- September 4, 2014, Member of the Scientific Organizing Committee; Presentation.
  • Bykova, N.V., Hu, J.-N., Rampitsch, C., Fan, T., and Radovanovic, N. (2014). "Quantitative comparative proteomic profiling of tissue-specific and membrane-associated seed proteomes in pre-harvest sprouting resistant and susceptible hybrid wheat genotypes.", 2nd Canadian Wheat Symposium, Saskatoon, SK, Canada, June 8-11, 2014. (Poster)
  • Bykova, N.V., Rampitsch, C., and others (2014). "Proteomic approaches to revealing mechanisms of physiological transition from seed dormancy to high germination potential in wheat.", Plant Biology 2014, Portland, Oregon, USA, July 12-16, 2014. (Poster)
  • Ma, Z., Marsolais, F., Igamberdiev, A.U., and Bykova, N.V. (2014). "Metabolism of organic acids in barley seeds during germination.", Plant Biology 2014, Portland, Oregon, USA, July 12-16, 2014, Presentation. (Poster)
  • Tailor, S., Bykova, N.V., and Debnath, S.C. (2014). "Genetic diversity in blueberry (Vaccinium spp. L.) using molecular markers.", Canadian Society of Agronomy & Canadian Society for Horticultural Science Joint Annual Conference, University of Lethbridge, Lethbridge, Alberta, Canada, July 10-12, 2014, pp. 33. (Abstract)

2013

  • Bykova, N.V., Hoehn, B., Rampitsch, C., Hu, J.-N., and Fan, T. (2013). "Redox-mediated regulation of wheat seed dormancy revealed through modification specific proteomics.", 11th International Conference on Reactive Oxygen and Nitrogen Species in Plant, Warsaw, Poland. (Poster)
  • Bykova, N.V., Hu, J.-N., Rampitsch, C., Stebbing, J.A., and Fan, T. (2013). "Proteomic profiling of membrane-associated protein complexes in wheat aleurone tissue using Blue Native gels and label-free semi-quantitative LC-MS/MS approach.", 5th Canadian National Proteomics Network Symposium, Vancouver, BC, Canada. (Poster)
  • Hu, J.-N., Stebbing, J.A., Fan, T., Rampitsch, C., and Bykova, N.V. (2013). "Proteomic analysis reveals changes in abundance, composition, and phosphorylation of membrane-associated protein complexes from wheat aleurone in response to alteration in seed dormancy.", CSPB Annual Meeting, Quebec City, Canada. (Poster)

2012

  • An, D., Bykova, N.V., and Debnath, S.C. (2012). "Identifying wild and cultivated cranberries and evaluating their relationships using morphology, and EST-PCR and ISSR markers.", Plant Canada 2012 (joint meeting of the Canadian Society of Agronomy (CSA), Canadian Society for Horticultural Science (CSHS), Certified Crop Advisors (CCA), North American Fruit Explorers (NAFEX)): Adapting Crops to Change, University of Saskatchewan, Saskatoon, SK, Canada, July 16-19, 2012, P1 Abstract Id 3764, pp. p.44.
  • Bykova, N.V. (2012). "Differential and redox-sensitive proteomic analysis of pre-harvest sprouting tolerance.", 1st Canadian Wheat Symposium, Winnipeg, MB, Canada, November 30-December 2, 2011, Published in: Can. J. Plant Sci., 92(3), pp. 600.
  • Bykova, N.V. and Igamberdiev, A.U. (2012). "Metabolism of reactive oxygen and nitrogen species during germination of barley seeds with different levels of dormancy.", Annual Meeting of the Canadian Society of Plant Physiologists (CSPP/SCPV 2012), University of Alberta Campus, Edmonton, AB, Canada, June 25-27, 2012. (Presentation)
  • Ma, Z., Igamberdiev, A.U., and Bykova, N.V. (2012). "Metabolism of reactive oxygen and nitrogen species during germination of barley seeds with different levels of dormancy.", Canadian Society of Plant Biologists Meeting (CSPB 2012), University of Alberta Campus, Edmonton, AB, Canada, June 25-27, 2012. (Presentation)
  • Shah, J.K., Hebelstrup, K.H., Bykova, N.V., and Igamberdiev, A.U. (2012). "The biotechnological use of hemoglobin overexpression in barley.", Annual Meeting of the American Society of Plant Biology, Austin, TX, USA, July 31-August 3, 2012, Poster P02048. (Abstract)

2011

  • Debnath, S.C., Siow, Y.L., Petkau, J.C., An, D., and Bykova, N.V. (2011). "Molecular markers and antioxidant activity in berry crop diversity analysis.", Plant Canada 2011, Saint Mary’s University, Halifax, NS, Canada, July 17-21, 2011, 78, Session 1. (Presentation)
  • Hu, J., Hoehn, B., Rampitsch, C., and Bykova, N.V. (2011). "Impact of dormancy genotypes on differential protein expression profiles and redox-sensitive landscape of the proteome in hybrid spring wheat lines.", Plant Canada 2011, Saint Mary’s University, Halifax, NS, Canada, July 17-21, 2011. (Poster)
  • Ma, Z., Igamberdiev, A.U., and Bykova, N.V. (2011). "The glycolytic and antioxidant H2O2 scavenging capacity of germinating barley seeds with different levels of dormancy.", Plant Canada 2011, Saint Mary’s University, Halifax, NS, Canada, July 17-21, 2011. (Presentation)

2010

  • Bykova, N.V., Hoehn, B., Rampitsch, C., Hu, J., Stebbing, J.A., and Knox, R.E. (2010). "Thiol redox-sensitive proteome and antioxidant protective mechanisms in wheat dormancy control.", Plant Biology 2010: Joint meeting of the Canadian Society of Plant Physiologists (CSPP) and American Society of Plant Biologists (ASPB), Montréal Convention Centre, Montréal, QC, Canada, July 31-August 4, 2010, P02045. (Abstract)
  • Igamberdiev, A.U., Bykova, N.V., and Kleczkowski, L.A. (2010). "Plant adaptation to low oxygen: switching to the alternative electron acceptor and the alternative energy currency.", Plant Biology 2010: Joint meeting of the Canadian Society of Plant Physiologists (CSPP) and American Society of Plant Biologists (ASPB), Montréal Convention Centre, Montréal, QC, Canada, July 31-August 4, 2010, P07033. (Abstract)

2009

  • Bykova, N.V. (2009). "Redox-Sensitive Proteome and Antioxidant Strategies in the innate immune response to Fusarium Graminearum in wheat.", 6th Canadian Workshop on Fusarium Head Blight (CWFHB) / Colloque canadien sur la fusariose, Marriott Ottawa, Ottawa, ON, Canada, November 1-4, 2009. (Presentation)
  • Bykova, N.V., Hoehn, B., Rampitsch, C., Banks, T.W., Stebbing, J.A., Fan, T., and Knox, R.E. (2009). "Redox-sensitive proteome and antioxidant strategies in wheat seed dormancy control.", Meeting on Plant Reactive Oxygen and Nitrogen Species, Helsinki, Finland, July 8-10, 2009, Abstract O15-118. (Presentation)
  • Igamberdiev, A.U., Bykova, N.V., and Hill, R.D. (2009). "Nitric oxide production and scavenging in plants under hypoxic conditions.", Meeting on Plant Reactive Oxygen and Nitrogen Species, Helsinki, Finland, July 8-10, 2009, Abstract O25-106. (Presentation)
  • Rampitsch, C., Subramaniam, R., Bykova, N.V., and Djuric-Ciganovic, S. (2009). "Protein phosphorylation and DON synthesis: Candidate phosphopeptides from wild-type and map kinase (mgv1)-deficient Fusarium graminearum.", 6th Canadian Workshop on Fusarium Head Blight (CWFHB) / Colloque canadien sur la fusariose, Marriott Ottawa, Ottawa, ON, Canada, November 1-4, 2009, P68. (Abstract)
  • Rampitsch, C., Subramaniam, R., Djuric-Ciganovic, S., and Bykova, N.V. (2009). "The phosphoproteome of Fusarium graminearum at the onset of nitrogen starvation reveals phosphopeptides from proteins with a potential role in mycotoxin regulation.", 11th International Congress on Amino Acids, Peptides and Proteins, Vienna, Austria, August 3-7, 2009, Abstract. Amino Acids 37: 112-113. (Abstract)
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