Hambleton, Sarah, Ph.D.

Research Scientist, Systematics of Rust and Smut Fungi

Contact Information:

K.W.Neatby Bldg. (#20 CEF)
960 Carling Ave
Ottawa, Ontario K1A 0C6
Telephone: 613-759-1769
Fax: 613-759-1701
Email: sarah.hambleton@agr.gc.ca

Areas of Expertise:

  • Systematic studies of rust and smut fungi on crop and weed hosts to improve identification systems and add to biodiversity records
  • Development of DNA sequence databases for rust and smut fungi from new collections and from herbarium specimens
  • Identifcation of species-specific molecular markers
  • Rapid detection and monitoring of high risk plant pathogenic fungi

Current Projects:

  • Detection and tracking of airborne rust spores as an information tool for pesticide-reduction strategies
  • PCR testing of soybean (Glycine max) in Ontario sentinel plots for presence of the Soybean Rust pathogens, Phakopsora spp.
  • Development of collections and reference / DND databases and detection systems to counter bioterrorism against agriculture and forestry.

Research publications

2017

2015

2014

2013

2012

2011

2010

2009

Other science related publications

2016

  • Hambleton, S., Eggertson, Q., Lévesque, C.A. Chen, W., Barasubiye, T., Redhead, S.A., and Liu, M. (2016). " DNA-barcoding the Powdery Mildews – sampling herbarium specimens in the National Mycological Herbarium (DAOM).", Canadian Phytopathological Society Annual Meeting, Delta Beausejour, Moncton, New Brunswick, Canada, June 12-15, 2016. (Poster)
  • Hambleton, S., Eggertson, Q., Wilson, S., Redhead, S.A., and Lévesque, C.A. (2016). "DNA-barcoding the rusts – sampling herbarium specimens in the National Mycological Herbarium (DAOM).", Canadian Phytopathological Society Annual Meeting, Delta Beausejour, Moncton, New Brunswick, Canada, June 12-15, 2016. (Abstract and Poster)

2015

  • Chen, W., Hambleton, S., and Lévesque, C.A. (2015). "Environmental metagenomics for plant pathogen surveillance: the promise and the practice.", Invited Oral Presentation at Institute of Microbiology, Chinese Academy of Sciences, Beijing, China, September 30, 2015. (Presentation)
  • Chen, W., Redhead, S.A., Seifert, K.A., Xiong, K., Hambleton, S., and Lévesque, C.A. (2015). "Fungal pathogen surveillance using metagenomics approach requires one fungus one name.", XVIII International Plant Protection Congress (IPPC), Free University Berlin, Henry Ford Building, Berlin, Germany, August 24-27, 2015.
  • Hambleton, S., Eggertson, Q.A., and Liu, M. (2015). "The rust pathogens of Rubus in Canada – identification, nomenclature and DNA-barcoding.", Botany 2015, Shaw Convention Center, Edmonton, Alberta, Canada, July 25-29, 2015.
  • Lévesque, C.A., Chen, W., Hambleton, S., Seifert, K.A., Adam, Z., Bilodeau, G.J., Cullis, J., Demeke, T., Dumonceaux, T.J., Gagnon, M., Graefenhan, T., Kawchuk, L.M., Khan, I.U.H., Lewis, C.T., Li, X., Links, M.G., Mimee, B., Rott, M., Tambong, J.T., Topp, E., Yu, Q., and Yuan, K. (2015). Project CRTI 09-462RD - Next generation sequencing, direct detection and genotyping of fungi, bacteria and nematodes in the agri-food system. DRDC-RDDC-2015-C148. Final Close-Out Report. (Report)
  • Lévesque, C.A., Chen, W., Hambleton, S., Seifert, K.A., Adam, Z., Bilodeau, G.J., Cullis, J., Demeke, T., Dumonceaux, T.J., Gagnon, M., Graefenhan, T., Kawchuk, L.M., Khan, I.U.H., Lewis, C.T., Li, X., Links, M.G., Mimee, B., Rott, M., Tambong, J.T., Yu, Q., and Yuan, K. (2015). Project CRTI 09-462RD - Next generation sequencing, direct detection and genotyping of fungi, bacteria and nematodes in the agri-food system. Final Report. 54. http://cradpdf.drdc-rddc.gc.ca/PDFS/unc195/p802330_A1b.pdf. (Report)
  • Lévesque, C.A., Chen, W., Hambleton, S., Seifert, K.A., and Graefenhan, T. (2015). "Environmental Sequencing – A New Frontier for Species Identification.", The First National Science Day of the Science and Technology Branch of AAFC, Horticulture Research and Development Centre (HRDC), St-Jean-sur-Richelieu, QC, Canada, July 9, 2015. (Presentation)
  • Lowe, C., Zahariev, M., Liu, M., Hambleton, S., Seifert, K.A., Lévesque, C.A., and Chen, W. (2015). "Computational pipeline to improve detection accuracy of plant pathogens from NGS datasets using signature oligonucleotides.", American Phytopathological Society Annual Meeting, Pasadena Convention Center, Pasadena, CA, USA, August 1-5, 2015. (Poster)

2014

  • Hambleton, S., Chen, W., Chapados, J.T., Lewis, C.T., and Lévesque, C.A. (2014). "Application of NGS technology in fungal pathogen detection and challenges in identification at species level.", 10th International Mycological Conference (IMC10), Bangkok, Thailand, August 3-8, 2014, Invited oral presentation.
  • Hambleton, S., Eggertson, Q.A., Edwards, T., Barasubiye, T., Redhead, S.A., and Lévesque, C.A. (2014). "DNA-barcoding herbarium specimens of obligate fungal pathogens – targeting the gaps in reference databases.", 10th International Mycological Conference (IMC10), Bangkok, Thailand, August 3-8, 2014. (Poster)
  • Hambleton, S., Temple, K., and Liu, M. (2014). "Taxonomy and DNA sequence variation of the rust fungi infecting four prairie grass species.", APS-CPS Joint Meeting, Hilton Minneapolis, Minneapolis, MN, USA, August 9-13, 2014. (Poster)
  • Kesanakurti, P., Richards, L., Gerdis, S., and Hambleton, S. (2014). "Developing a DNA-based toolkit for detection and identification of wheat rust pathogens.", APS-CPS Joint Meeting, Hilton Minneapolis, Minneapolis, MN, USA, August 9-13, 2014. (Poster)

2013

  • Fung, S., Rempel, H., Block, G.S., Chen, W., Janzen, T., Lewis, C.T., Zhang, N., El-Kayssi, N., Bilkhu, S., Hambleton, S., Amoako, K.K., Lévesque, C.A., Bach, S.J., Delaquis, P.J., and Diarra, M.S. (2013). "Bacillus species across Canada's agriculture environment: an ecological perspective.", International conference on Bacillus antracis, B. cereus, and B. thurengiensis, Victoria, BC, Canada, September 1-5, 2013, Abstract. (Poster)
  • Kesanakurti, P., Setia, R., Temple, K., Hambleton, S., Lévesque, C.A., and Lewis, C.T. (2013). "Genome data mining and diagnostic marker development of Tilletia indica for Agri-food system detection screening.", Phytopathology, 103(Suppl.2:S2), pp. 73. (Abstract)
  • Lévesque, C.A., Lewis, C.T., Assabgui, R., McCormick, W.A., Ponovera, E., Hambleton, S., and Seifert, K.A. (2013 internal). "Development of additional DNA barcodes for Fungi based on conserved Pfam families and domains.", 21st Annual Conference of Intelligent Systems for Molecular Biology (ISMB) and European Conference on Computational Biology (ECCB), Berlin, Germany, July 19-23, 2013, Invited oral presentation.
  • Lewis, C.T., Chen, W., Zhang, N., El-Kayssi, N., Fung, S., Diarra, M.S., Bilkhu, S., Hambleton, S., and Lévesque, C.A. (2013). "Developing an integrated bioinformatics platform to support real-time monitoring of agricultural environments.", 21st Annual Conference of Intelligent Systems for Molecular Biology (ISMB) and European Conference on Computational Biology (ECCB), Berlin, Germany, July 19-23, 2013.
  • Malloch, D., Sigler, L., and Hambleton, S. (2013). "Leuconeurospora capsici comb. nov. and Leuconeuorspora polypaeciloides sp. nov. - New Species Name registration.", Index Fungorum, 20.
  • Ordoñez, M.E., Vivvanco, C.E., Hambleton, S., and Barnes, C.W. (2013). "Puccinia on wheat and other Poaceae in the Ecuadorian highlands.", Phytopathology, 103(Suppl.2:S2), pp. 108. (Abstract)
  • Sigler, L., Hambleton, S., and Paré, J.A. (2013). "Ophidiomyces gen. nov. and Ophidiomyces ophiodiicola comb. nov. - New Species Name registration.", Index Fungorum, 19.

2012

  • Chen, W., Lewis, C.T., Chapados, J.T., Hambleton, S., Seifert, K.A., Temple, K., Biernacka-Larocque, A., Robleh Djama, Z., and Lévesque, C.A. (2012). "Canada-wide spore-trapping network provides effective monitoring of microbial phytopathogens in air and rain samples.", Annual meeting of the American Phytopathological Society, Providence, RI, USA, August 4-8, 2012, In: Phytopathology 102: S4.22.
  • Lévesque, C.A., Bilkhu, S., Chapados, J.T., Chen, W., Hambleton, S., Lewis, C.T., Oliver, K., and Seifert, K.A. (2012). "AeroNet: A Canadian spore trapping network monitoring fungi and bacteria with DNA barcodes and next generation sequencing.", The Role of Genomics & Bioinformatics in Protecting Canadians from Microbial Food Safety Risks Workshop, Southway Hotel and Conference Centre, Ottawa, ON, Canada, February 21-22, 2012, Invited oral presentation.
  • Rintoul, T.L., Rintoul, T., Lévesque, C.A., Robideau, G.P., Eggertson, Q.A., Rodrigue, N., Chen, W., Spies, C.F.J., Bala, K., de Cock, A.W.A.M., Coffey, M.F., Hamilton, J.P., Hambleton, S., Adhikari, B., and Buell, C.R. (2012). "Molecular diagnosis, identification and phylogeny of Pythium, Phytopythium and related genera.", 4th International Workshop on Phytophthora, Pythium and Phytopythium, College Park, MD, USA, May 21-25, 2012, Invited oral presentation.
  • Seifert, K.A., Graefenhan, T., Chen, W., Lewis, C.T., Bilkhu, S., Chapados, J.T., Hambleton, S., and Lévesque, C.A. (2012). "Prospects for using quantitative PCR and metagenomics to detect mycotoxigenic fungi without culturing.", MycoRed International Conference, Carleton University, Ottawa, ON, Canada, June 2012.

2011

  • Chen, W., Lewis, C.T., Chapados, J.T., Bilkhu, S., Hambleton, S., Seifert, K.A., and Lévesque, C.A. (2011). "Aerobiota diversity in Canada’s air: Mining the metagenomic data.", Great Lakes - St. Lawrence Spring Workshop in Mycology, Peterborough, ON, Canada, May 7-8, 2011. (Presentation)
  • Hambleton, S., Chen, W., Lewis, C.T., Bilkhu, S., Chapados, J.T., Oliver, K., Tenuta, A.U., and Lévesque, C.A. (2011). "AeroNet Canada: Profiling rust fungus diversity in spore trap samples with DNA barcoding and next generation sequencing.", North American Rust Workers Meeting (NARW), St. Paul, MN, USA, June 12, 2011. (Presentation)
  • Lévesque, C.A., Bilkhu, S., Chapados, J.T., Chen, W., Hambleton, S., Lewis, C.T., Oliver, K., and Seifert, K.A. (2011). "AeroNet: A Canadian spore trapping network monitoring fungi and bacteria with DNA barcodes and next generation sequencing.", iBOL-Canada: The future of DNA Barcoding in Canada, Beaty Biodiversity Museum, UBC, Vancouver, BC, Canada, April 29-May 1, 2011. (Presentation)
  • Marsh, K., McCallum, B.D., Wang, X., Tenuta, A.U., Hambleton, S., and Saville, B. (2011). "Investigating emerging wheat pathogens, new wheat leaf rust virulence in Ontario.", 1st Canadian Wheat Symposium, Winnipeg, MB, Canada, November 30-December 2, 2011.
  • Tsuneda, A., Hambleton, S., and Currah, R.S. (2011). "The anamorph genus Knufia and its phylogenetically allied species in Coniosporium, Sarcinomyces, and Phaeococcomyces., [Botany/Botanique, 89(8), pp. 523-536.] (Corrigendum).", Botany, 89(12), pp. 887.

2009

  • Lévesque, C.A., Seifert, K.A., Babcock, C., Bissett, J.D., Hambleton, S., Lewis, C.T., Redhead, S.A., Brière, S.C., Côté, M.J., and Hamelin, R.C. (2009). "CRTI-04-0045RD - Development of collections, reference/DNA databases and detection systems to counter bioterrorism against agriculture and forestry.", 7th Annual CRTI Summer Symposium 2009, Ottawa, ON, Canada, June 16-18, 2009, pp. 10-11.
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