Ouellet, Thérèse, Ph.D.

Research Scientist, molecular pathology in cereals

Contact Information:

960 Carling Ave
Ottawa, Ontario K1A 0C6
Telephone: 613-759-1658
Fax: 613-759-1701
Email: therese.ouellet@agr.gc.ca

Areas of Expertise:

  • Understanding molecular mechanisms of disease resistance to Fusarium head blight in wheat through Genomics studies
  • Understanding molecular mechanisms of disease resistance to pink ear rot in maize
  • Understanding molecular mechanisms of pathogenecity of Fusarium graminearum on cereals
  • Identification of gene regulatory elements that modulate gene expression in plants

Current Projects:

  • A Systems Biology Approach to Dissecting the Fusarium/Cereal Interaction: Reduction of Mycotoxins in Food and Feed
  • Proteome and Transcriptome Analyses of FHB resistance in winter wheat
  • Fine mapping of individual FHB resistance genes and correlation with changes in gene expression profiles in wheat.
  • Characterization of the mechanisms of defence in novel sources of resistance to FHB

Affiliations:

  • Adjunct Professor, University of Ottawa, Biology department

Research publications

2016

2015

2014

2013

  • Harris, L.J., Ouellet, T., and Subramaniam, R. (2013). "Applying proteomics to investigate the interactions between pathogenic Fusarium species and their hosts.", in Brown, D.W. and Proctor, R.H. (eds.) - Fusarium: Genomics, Molecular and Cellular Biology, Caister Academic Press, Chapter 5.

2012

2011

2010

2009

Other science related publications

2017

  • Hu, X., McCartney, C., Biselli, C., Rocheleau, H., Balcerzak, M., Fedak, G., Khanizadeh, S., Yan, Z., Valè, G., Ouellet, T. 2017. Expression markers closely associated with the 2DL QTL locus for fusarium head blight resistance. Proceedings of the 13th International Wheat Genetics Symposium, Tulln, Austria, April 23-28, 2017. P. 236.
  • Pan Y, Li Y, Liu Z, Surendra A, Wang L, Foroud NA, Goyal RK, Ouellet T, Fobert PR. Differential expression feature extraction and its application in wheat RNA-seq data analysis related with FHB. Canadian Wheat Alliance Science and Business Meeting. Saskatoon SK, CANADA, November 8-9, 2017. Poster Presentation.

2016

  • Eranthodi, A., Subramaniam, R., Ouellet, T., Rampitsch, C., Foroud, N.A., and Schultz, E.A. (2016). "Mutant-based screening for pathogen-associated molecular patterns involved in Fusarium head blight disease resistance in wheat.", Prairie University Biology Symposium 2016, The University of Lethbridge, Lethbridge, AB, Canada, February 18-20, 2016. (Poster)
  • Liu, Z., Li, Y., Pan, Y., Wang, L., Ouellet, T. and Fobert, P. 2016. Improvement of wheat-fusarium dual genome RNA-seq data processing. Third Annual Science Meeting of the Canadian Wheat Alliance, Saskatoon, June 27-28, 2016.
  • Ouellet, T., Gou, L., Konkin, D., Hattori, J., Tekieh, F., Wolfe, D.F., Balcerzak, M., Chalabian, F., Vrana, J., Kubaláková, M., Doležel, J., Tinker, N.A., Sharpe, A.G., and Fedak, G. (2016). "Towards cloning of the FHB-resistance locus from Thinopyrum elongatum chromosome 7EL.", XIV Plant and Animal Genome Conference, San Diego, CA, USA, January 2016. (Poster)
  • Pan, Y., Li, Y., Liu, Z., Surendra, A., Wang, L., Foroud, N., Ouellet, T., Fobert, F. 2016. Differential Gene Expression Feature Extraction from FHB Challenged Wheat RNA-seq Data. Proceedings of the 8th Canadian Workshop on Fusarium Head Blight, Ottawa, ON, November 20-22 2016. P. 53.
  • Tulpan, D., Léger, S., Konkin, D. and Ouellet, T. 2016. Visualisation of orthology-based plant genome zipper seeding using circular diagrams. Third Annual Science Meeting of the Canadian Wheat Alliance, Saskatoon, June 27-28, 2016.
  • Wang, L., Forseille, L., Boyle, K., Francis, T., Pan, Y., Liu, Z., Li, Y., Zaharia, L.I., Ouellet, T. and Fobert, P. 2016. Molecular analysis of novel FHB resistance. Third Annual Science Meeting of the Canadian Wheat Alliance, Saskatoon, June 27-28, 2016.
  • Wang, Y., Pan, Y., Richard, R., and Ouellet, T. 2016. Identification of Fusarium head blight responsive gene modules and genetic markers by meat-analysis of transcriptome datasets in wheat. Third Annual Science Meeting of the Canadian Wheat Alliance, Saskatoon, June 27-28, 2016.

2015

  • Eranthodi, A., Subramaniam, R., Ouellet, T., Rampitsch, C., Schultz, E.A., and Foroud, N.A. (2015). "Fusarium graminearum mutant screening towards identification of pathogen-associated molecular patterns in the Fusarium head blight-wheat interaction.", 36th Annual Meeting of the Plant Pathology Society of Alberta, Lethbridge, AB, Canada, November 16-18, 2015, Oral Presentation.
  • Eranthodi, A., Subramaniam, R., Ouellet, T., Rampitsch, C., Schultz, E.A., and Foroud, N.A. (2015). "Towards the identification and characterization of pathogen-associated molecular patterns from the Fusarium graminearum secretome.", 1st Lethbridge Research Centre Graduate Student Symposium, Lethbridge, AB, Canada, November 26, 2015, Award Winning: 2nd Place for Oral Presentation.
  • Eranthodi, A., Subramaniam, R., Rampitsch, C., Ouellet, T., Schultz, E.A., and Foroud, N.A. (2015). "Generation of Fusarium graminearum mutants to screen candidate pathogen-associated molecular patterns.", National Fusarium Head Blight Forum, St. Louis, MO, USA, December 6-8, 2015. (Poster)
  • Ouellet, T. et al. (2015). Gene expression during early infection by Fusarium graminearum of wheat lines susceptible and resistant to fusarium head blight. GEO accession #GSE54556, deposited in the Gene Expression Omnibus (GEO) of the public database NCBI. This accession is a SuperSeries that contain expression data for 7 separate gene expression experiments, including gene expression for the susceptible wheat cultivar Roblin when inoculated with F. graminearum strains producing or not DON, the resistant cultivars Wuhan 1, NuyBay and Dream, and 7 breeding lines segregating for 3 fusarium head blight resistance QTLs. Accession numbers for the individual experiments are GSE36283 and GSE54550 to GSE54555.
  • Ouellet, T. et al. (2015). Gene expression in wheat heads treated with Fusarium graminearum mutants that produce a priming-induced resistance to fusarium head blight. GEO accession #GSE69509, deposited in the Gene Expression Omnibus (GEO) of the public database NCBI.
  • Ouellet, T., Rocheleau, H., and Subramaniam, R. (2015). Gene expression in wheat heads treated with the mutant tri6Δ of Fusarium graminearum, which produces a priming-induced resistance to fusarium head blight. GEO accession #GSE69437, deposited in the Gene Expression Omnibus (GEO) of the public database NCBI.
  • Pan, Y., Ouellet, T., Fauteux, F., Tulpan, D., Tchagang, A., and Phan, S. (2015). "Analysis of hub genes associated with wheat FHB defense.", 2nd Annual Science Meeting of the Canadian Wheat Association, Saskatoon, Canada, July 14-16, 2015. (Poster)
  • Pan, Y., Ouellet, T., Phan, S., Tchagang, A., Fauteux, F., and Tulpan, D. (2015). "Digitization of Trait Representation in Microarray Data Analysis of Wheat Infected by Fusarium graminearum.", Computational Intelligence in Bioinformatics and Computational Biology, C-15050.
  • Ravensdale, M., Wang, L., Rocheleau, H., Ouellet, T., and Subramaniam, R. (2015). Gene expression during priming-induced resistance to fusarium head blight in wheat as revealed by two distinct mutants of Fusarium graminearum. GEO accession #GSE55653, deposited in the Gene Expression Omnibus (GEO) of the public database NCBI.
  • Subramaniam, R., Wang, L., and Ouellet, T. (2015). Gene expression in wheat heads treated with the mutant noxABΔ of Fusarium graminearum, which produces a priming-induced resistance to fusarium head blight. GEO accession #GSE69490, deposited in the Gene Expression Omnibus (GEO) of the public database NCBI.

2014

  • Fedak, G., Wolfe, D., Chi, D., Ouellet, T., Cao, W., and Xue, A.G. (2014). "Process of transferring fhb resistance from thinopyrum elongatum to spring wheat.", 2nd Canadian Wheat Symposium, Saskatoon, SK, Canada, June 8-11, 2014. (Poster)
  • Ouellet, T. and Wang, L. (2014). Gene expression during early infection of the susceptible spring wheat cultivar Roblin with Fusarium graminearum, the major causal agent of fusarium head blight in wheat. GEO accession #GSE36283, deposited in the Gene Expression Omnibus (GEO) of the public database NCBI.
  • Ouellet, T., Balcerzak, M., Leung, W.W.Y., Martin, T., and Gulden, S.M. (2014). "DON induces genes that increase wheat susceptibility to Fusarium head blight in wheat.", 2nd Canadian Wheat Symposium, Saskatoon, SK, Canada, June 8-11, 2014. (Poster)

2013

  • Ouellet, T., Balcerzak, M., Leung, W.W.Y., Hattori, J., Martin, T., Gulden, S.M., Wójcik, P.I., Dzwinel, W. (2013). "DON Induces Genes that Increase Wheat Susceptibility to Fusarium Head Blight in Wheat.", National Fusarium Head Blight Forum, Milwaukee, WI, USA, December 3-5, 2013, pp. 76.

2012

  • Hattori, J., Chao, S., Ouellet, T., Jellen, E.N., Jackson, E.W., Oliver, R.E., Lazo, G.R., and Tinker, N.A. (2012). "Inter-genome distances in hexaploid oat and wheat estimated from single copy candidate genes.", 9th International Oat Conference: Harvest for Food and Health, Beijing, China, June 20-23, 2012. (Poster)
  • Ouellet, T., Balcerzak, M., Leung, W.W.Y., Hattori, J., Martin, T., and Gulden, S.M. (2012). "The mycotoxin, Deoxynivalenol, induces genes that increase susceptibility to fusarium head blight in wheat.", 10th International Plant Molecular Biology (IPMB) Congress, Jeju, South Korea, October 21-26, 2012. (Poster)

2011

  • Allard, G.C., Subramaniam, R., Ouellet, T., Harris, L.J., and Singh, J. (2011). "Rapid screening of Arabidopsis mutants to identify transporter genes for resistance to Fusarium graminearum.", 7th Canadian Workshop on Fusarium Head Blight (CWFHB) / Colloque canadien sur la fusariose, Delta Winnipeg Hotel, Winnipeg, MB, Canada, November 27-30, 2011, pp. 100.
  • Fedak, G., Cao, W., Chi, D., Somers, D.J., Miller, S.S., Ouellet, T., Xue, A.G., Gilbert, J., Savard, M.E., and Voldeng, H.D. (2011). "New sources of resistance to fusarium head blight and their modes of action.", 2011 National Fusarium Head Blight Forum, Hyatt Regency Hotel, St. Louis, MO, USA, December 4-6, 2011, pp. 19.
  • Foroud, N.A., Ouellet, T., Ellis, B.E., Jordan, M.C., Laroche, A., and Eudes, A. (2011). "Signalling mechanisms mediating resistance and susceptibility to fusarium head blight in wheat.", 7th Canadian Workshop on Fusarium Head Blight (CWFHB) / Colloque canadien sur la fusariose, Delta Winnipeg Hotel, Winnipeg, MB, Canada, November 27-30, 2011, pp. 53.
  • Ouellet, T., Balcerzak, M., Martin, T., and Gulden, S.M. (2011). "Wheat genes induced by DON are associated with susceptibility to fusarium head blight (FHB).", 7th Canadian Workshop on Fusarium Head Blight (CWFHB) / Colloque canadien sur la fusariose, Delta Winnipeg Hotel, Winnipeg, MB, Canada, November 27-30, 2011, pp. 54.
  • Subramaniam, R., Nasmith, C.G., Ouellet, T., Wang, L., and Rocheleau, H.J. (2011). "Novel strategies to combat fusarium head blight.", 7th Canadian Workshop on Fusarium Head Blight (CWFHB) / Colloque canadien sur la fusariose, Delta Winnipeg Hotel, Winnipeg, MB, Canada, November 27-30, 2011, pp. 64.

2010

  • Balcerzak, M., Schneiderman, D., Johnson, A., Harris, L.J., and Ouellet, T. (2010). "Transcriptome Analysis Of Fusarium Graminearum During Infection of Three Monocot Hosts.", 11th European Fusarium Seminar: Fusarium - Mycotoxins, Taxonomy, Pathogenicity and Host Resistance, Plant Breeding and Acclimatization Institute (IHAR), Radzików, Poland, September 20-23, 2010, Poster #1. (Poster)
  • DesRoches, C., Balcerzak, M., Blackwell, B.A., Johnston, A., Harris, L.J., and Ouellet, T. (2010). "Investigating the Pathways and Genes Responsible for the Production of Indole-3-Acetic Acid by {I}Fusarium Graminearum{/I}.", 11th European Fusarium Seminar: Fusarium - Mycotoxins, Taxonomy, Pathogenicity and Host Resistance, Plant Breeding and Acclimatization Institute (IHAR), Radzików, Poland, September 20-23, 2010, Poster #51. (Poster)
  • Foroud, N.A., Ellis, B.E., Ouellet, T., Friedt, J., Oosterveen, B., Jordan, M.C., Laroche, A., and Eudes, F. (2010). "Investigating the molecular mechanisms of fusarium head blight resistance in wheat.", 31st Annual Meeting of the Plant Pathology Society of Alberta, Lethbridge, AB, Canada, October 12-14, 2010. (Presentation)
  • Gaudet, D.A., Wang, Y., Frick, M., Penniket, C., Puchalski, B., Ouellet, T., Singh, J., Robert, L.S., and Laroche, A. (2010). "Cold-induced snow mould resistance in winter wheat.", ESA-PPSA-WFPM, Lethbridge, AB, Canada, pp. 20.
  • Miller, S.S. and Ouellet, T. (2010 Internal). Characterization of the mechanisms of defence expressed in the rachis of novel sources of resistance to FHB. Interim report to Ontario Wheat Producer's Marketing Board., AAFC Eastern Cereal and Oilseed Research Centre, Ottawa, ON. (Report)

2009

  • Balcerzak, M., Gulden, S.M., and Ouellet, T. (2009). "Comparative analysis of the wheat spikelet transcriptome after inoculation with DON+ and DON- strains of Fusarium graminearum.", 14th International Congress on Molecular Plant-Microbe Interactions, Centre des congrès de Québec, Québec City, QC, Canada, July 19-23, 2009. (Poster)
  • Balcerzak, M., Gulden, S.M., and Ouellet, T. (2009). "Expression profiling of trichothecene induced genes in wheat.", 6th Canadian Workshop on Fusarium Head Blight (CWFHB) / Colloque canadien sur la fusariose, Marriott Ottawa, Ottawa, ON, Canada, November 1-4, 2009, pp. 89. (Poster)
  • DesRoches, C., Balcerzak, M., Blackwell, B.A., and Ouellet, T. (2009). "Investigating the role of the production of auxins by Fusarium graminearum.", 6th Canadian Workshop on Fusarium Head Blight (CWFHB) / Colloque canadien sur la fusariose, Marriott Ottawa, Ottawa, ON, Canada, November 1-4, 2009, pp. 91. (Poster)
  • Gulden, S.M., Watson, E.M., Frégeau-Reid, J.A., Miller, S.S., Fedak, G., Ouellet, T. (2009). "Characterization of a novel resistance to Fusarium graminearum identified in the grass Thinopyrum elongatum.", 14th International Congress on Molecular Plant-Microbe Interactions, Centre des congrès de Québec, Québec City, QC, Canada, July 19-23, 2009. (Poster)
  • Gulden, S.M., Watson, E.M., Miller, S.S., and Ouellet, T. (2009). "Thinopyrum elongatum as a novel source of FHB resistance.", 6th Canadian Workshop on Fusarium Head Blight (CWFHB) / Colloque canadien sur la fusariose, Marriott Ottawa, Ottawa, ON, Canada, November 1-4, 2009, pp. 89. (Poster)
  • Miller, S.S. and Ouellet, T. (2009 Internal). Characterization of the mechanisms of defence expressed in the rachis of novel sources of resistance to FHB. Interim report to Ontario Wheat Producer's Marketing Board., AAFC Eastern Cereal and Oilseed Research Centre, Ottawa, ON. (Report)
  • Ouellet, T., Hattori, J., Gulden, S.M., Balcerzak, M., Rocheleau, H.J., Wang, L., Singh, J., Foroud, N.A., Fedak, G., Pandeya, R.S., Somers, D.J., Eudes, F., and Tinker, N.A. (2009). "Expression profiling of FHB resistant wheat: what we have learned from it so far.", 6th Canadian Workshop on Fusarium Head Blight (CWFHB) / Colloque canadien sur la fusariose, Marriott Ottawa, Ottawa, ON, Canada, November 1-4, 2009, pp. 103. (Workshop)
  • Qi, P.-F., Wei, Y.-M., Ouellet, T., and Zheng, Y.-L. (2009). "Salicylic acid inhibits the germination and growth of Fusarium graminearum and improves the resistance of wheat to Fusarium Head Blight.", 6th Canadian Workshop on Fusarium Head Blight (CWFHB) / Colloque canadien sur la fusariose, Marriott Ottawa, Ottawa, ON, Canada, November 1-4, 2009, pp. 102. (Poster)
  • Rampitsch, C., Somers, D.J., Tamburic-Ilincic, L., Fedak, G., Ouellet, T., Humphreys, D.G., and DePauw, R.M. (2009). "Strategic application of proteomics and plant genotyping toward improved FHB resistance in Canadian wheat.", GABI 9th Statusseminar, Potsdam, Germany, March 3-5, 2009. (Poster)
  • Rocheleau, H.J., Zheng, W., Gulden, S.M., Xu, R., Wang, L., and Ouellet, T. (2009). "Comparative gene expression profiling of major plant hormone pathways during infection by Fusarium graminearum.", 6th Canadian Workshop on Fusarium Head Blight (CWFHB) / Colloque canadien sur la fusariose, Marriott Ottawa, Ottawa, ON, Canada, November 1-4, 2009, pp. 103. (Poster)
  • Rocheleau, H.J., Zheng, W., Gulden, S.M., Xu, R., Wang, L., and Ouellet, T. (2009). "Validation of expression profiling patterns for plant hormone signalling pathways in wheat infected by Fusarium graminearum.", 14th International Congress on Molecular Plant-Microbe Interactions, Centre des congrès de Québec, Québec City, QC, Canada, July 19-23, 2009. (Poster)
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