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Pseudomonas canadensis sp. nov., a biological control agent isolated from a field plot under long-term mineral fertilization

James T. Tambong, Renlin Xu and Eden Bromfield (2016) Pseudomonas canadensis sp. nov., a biological control agent isolated from a field plot under long-term mineral fertilization. International Journal of Systematic and Evolutionary Microbiology doi:10.1099/ijsem.0.001698.  Access to full text

Abstract

The bacterial strain 2-92T isolated from a field plot under long-term (>40 years) mineral fertilization exhibited in vitro antagonistic properties against fungal pathogens. A polyphasic approach was undertaken to verify its taxonomic status. Strain 2-92T is gram-negative, aerobic, non-spore-forming, motile by one or more flagella, and oxidase-, catalase- and urease-positive. Optimal growth temperature of strain 2-92T is 30 °C. 16S rRNA gene sequence analysis demonstrated that the strain is related to species of the genus Pseudomonas. Phylogenetic analysis of six housekeeping genes (dnaA, gyrB, recA, recF, rpoB and rpoD) revealed that strain 2-92T clustered as a distinct and well separated lineage with P. simiae as its most closely-related species. Polar lipid and fatty acid composition corroborated the taxonomic position of strain 2-92T in the genus Pseudomonas. Phenotypic characteristics from carbon utilizations could be used to differentiate strain 2-92T from closely related species of Pseudomonas. DNA-DNA hybridization values (wet laboratory and genome-based) and average nucleotide identity data confirmed that this strain represents a novel species. On the basis of phenotypic and genotypic characteristics, it is concluded that this strain represents a separate novel species for which the name Pseudomonas canadensis sp. nov. is proposed, with type strain 2-92T (= LMG 28499T = DOAB 798T). The G+C content is 60.30 mol%.

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