Microsatellite analysis of chromosomes from the fifth homoeologous group in the introgressive Triticum aestivum/Amblyopyrum muticum wheat lines.

Iefimenko, T.S., Fedak, G., Antonyuk, M.Z., and Ternovska, T.K. (2015). "Microsatellite analysis of chromosomes from the fifth homoeologous group in the introgressive Triticum aestivum/Amblyopyrum muticum wheat lines.", Cytology and Genetics, 49(3), pp. 183-191.

Abstract

Introgressive hybridization of common wheat has been and remains an efficient way for enriching its gene pool with resistance genes against environmental stress factors. In this aspect, T genome of the diploid species Amblyopyrum muticum (Boiss.) Eig. is believed to be prospective. The genome substitution amphidiploid Aurotica (AABBTT) with the A and B subgenomes from the common wheat cultivar Aurora and with the T subgenome from the indicated species is characterized by a high level of winter resistance. This trait is associated in wheat with the chromosomes of the fifth homoeologous group. Using a comparative microsatellite analysis of Aurora and Aurotica DNAs, we identified nine SSR loci specific for the wheat D genome, whose primers produced different amplicons from Aurotica DNA. The latter were used in screening F5 plants (Aurora × Aurotica) for the search of the specimens that carried the 5T chromosome or its fragments in their genomes. Among 234 plants, we detected 24 plants with disomic 5D/5T substitutions, which were the progeny of five F4 plants grown from two F2 plants. A significant number of plants contained the recombinant 5D/5T chromosome. The recombination event could occur in any generation starting with F2.

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