Anti-inflammatory and anti-chemotactic effects of dietary flaxseed oil on CD8+ T cell/adipocyte-mediated cross-talk.
Monk, J.M., Liddle, D.M., Brown, M., Zarepoor, L., De Boer, A.A., Ma, D.W.L., Power, K.A., and Robinson, L.E. (2015). "Anti-inflammatory and anti-chemotactic effects of dietary flaxseed oil on CD8+ T cell/adipocyte-mediated cross-talk.", Molecular Nutrition and Food Research. doi : 10.1002/mnfr.201500541 Access to full text
Scope: CD8+ T cell/adipocyte paracrine interactions represent a critical step in the development of the obese inflammatory phenotype that is disrupted by long chain n-3 PUFA. Our objective was to determine the effect of flaxseed-derived n-3 PUFA (α-linolenic acid) on these paracrine interactions. Methods and results: C57BL/6 mice were fed a 3.5% flaxseed oil + 3.5% corn oil (FX) diet (w/w) or an isocaloric 7% corn oil (w/w) control diet (CON) for 3 weeks. 3T3-L1 adipocytes and purified primary splenic CD8+ T cells were co-cultured at an obese cellular ratio (10% CD8+ T cells) and LPS-stimulated (10 ng/mL mimicking obese circulating endotoxin levels) for 24 h. FX co-cultures reduced i) secreted IL-6, TNFα, MCP-1, MIP-1α, and RANTES levels, ii) activation of inflammatory transcription factors NFκB p65 and STAT3 and iii) RAW264.7 macrophage chemotaxis versus CON (P≤0.05). Co-culture of pre-inflamed adipocytes (10 ng/mL LPS, 24 h prior to CD8+ T cell addition) resulted in reduced secretion of IL-6, IL-1β, MCP-1, MCP-3, MIP-1β and RANTES in FX co-cultures versus CON (P≤0.05). Conclusion: FX exerts an anti-chemotactic and anti-inflammatory effect on CD8+ T cell/adipocyte paracrine interactions (cross-talk), which has the potential to mitigate macrophage chemotaxis which drives components of the obese phenotype.
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