Supplementing Propionibacterium acidipropionici P169 does not affect methane production or volatile fatty acid profiles of different diets in in vitro rumen cultures from heifers.
Alazzeh, A.Y., Smith, A.H., Beauchemin, K.A., Meale, S.J., and McAllister, T.A. (2015). "Supplementing Propionibacterium acidipropionici P169 does not affect methane production or volatile fatty acid profiles of different diets in in vitro rumen cultures from heifers.", Acta Agriculturae Scandinavica, Section A - Animal Science, 64(3), pp. 170-177. doi : 10.1080/09064702.2014.988746 Access to full text
Effects of Propionibacterium acidipropionici P169 on gas, methane (CH4) production and volatile fatty acids in in vitro rumen cultures were studied. Ground barley (Experiment 1), mixed forage (Experiment 2) or mixed concentrate-forage (Experiment 3) served as substrates. Three doses of P169 were used in Experiments 1 and 2 [1.2 × 104; 1.2 × 106; 1.2 × 108 colony forming unit (CFU)/mL], while two doses (2.4 × 106; 2.4 × 108 CFU/mL) were used in Experiment 3. In Experiment 1, P169 increased (P < 0.05) gas production at 6–12 h and CH4 (mL/g diet dry matter) at all times. In Experiment 2, CH4 was increased at 6 h (P < 0.01). At 12 h, propionate declined (P < 0.01) in Experiment 1 and increased at 48 h in Experiment 3. The inability of P169 to increase propionate and its lack of persistence in mixed ruminal cultures may account for its inability to alter CH4 production.
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