Ultrafiltration LC-ESI-MSn screening of MMP-2 inhibitors from selected Chinese medicinal herbs Smilax glabra Roxb., Smilax china L. and Saposhnikovia divaricata (Turcz.) Schischk as potential functional food ingredients.

Li, L., Li, B., Zhang, H., Zhao, A., Han, B., Liu, C.M., and Tsao, R. (2015). "Ultrafiltration LC-ESI-MSn screening of MMP-2 inhibitors from selected Chinese medicinal herbs Smilax glabra Roxb., Smilax china L. and Saposhnikovia divaricata (Turcz.) Schischk as potential functional food ingredients.", Journal of Functional Foods, 15, pp. 389-395. doi : 10.1016/j.jff.2015.03.038  Access to full text

Abstract

In vitro Matrix metalloproteases-2 (MMP-2) inhibition assay and ultrafiltration liquid chromatography coupled to electrospray ionization tandem mass spectrometry (ultrafiltration LC-ESI-MSn) were combined to screen MMP-2 inhibitors from three Chinese medicinal herbs, Smilax glabra Roxb., Smilax china L. and Saposhnikovia divaricata (Turcz.) Schischk as potential functional food ingredients. Results showed that extracts of two Smilax species and the three major components resveratrol, engelitin and asibinn, and extract of S.divaricata and its major component 4′-O-β-D-glucosyl-5-O-methylvisamminol and three other chromones were strong MMP-2 inhibitors. Resveratrol and 4′-O-β-D-glucosyl-5-O-methylvisamminol had the highest binding affinity to MMP-2 and were the main contributors to the MMP-2 activity of Smilax and Saposhnikovia extracts, respectively. The present results further suggest that ultrafiltration LC-ESI-MSn is a valid, selective and effective method, and can be used to screen MMP-2 inhibitors from other plants such as grapes that contain resveratrol as functional foods and nutraceuticals.

Date modified: