Analysis of complete genome sequences of swine hepatitis E virus and V239A substitution in helicase domain of genotype 3 hepatitis E virus strains isolated from human and swine in Canada.

Ward, P., Leblanc, D., Houde, A., and Brassard, J. (2015). "Analysis of complete genome sequences of swine hepatitis E virus and V239A substitution in helicase domain of genotype 3 hepatitis E virus strains isolated from human and swine in Canada.", Archives of Virology, 160(7), pp. 1767-1773. doi : 10.1007/s00705-015-2429-8  Access to full text

Abstract

Among Canadian swine HEV strains, only one complete genome sequence has been published so far, and there are no data on the virulence of these strains. A collection of 28 Canadian swine HEV strains was used in this study. After RNA extraction, a portion of ORF2, the 3’ end of the helicase domain, and two complete genomes were amplified and sequenced. These two new Canadian complete genomes belonged to two different subtypes and showed 87.5 and 87.7 % sequence identity to the Canadian swine HEV strain Arkell. The V239A substitution within the helicase domain, which is associated with increased virulence of genotype 3 HEV, was detected in one Canadian swine HEV strain. However, no human hepatitis E infections have been associated with this strain.

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