Quick test for qualitative detection of glyphosate-resistant kochia (Kochia scoparia L. Schrad.).

Pratchler, J., Shirriff, S., and Beckie, H.J. (2014). "Quick test for qualitative detection of glyphosate-resistant kochia (Kochia scoparia L. Schrad.).", Canadian Journal of Plant Science, 94(8), pp. 1413-1417. doi : 10.4141/cjps-2014-180  Access to full text

Abstract

A growth chamber study was conducted to determine the feasibility of developing a quick, yet reliable assay to detect glyphosate-resistant (GR) kochia. Seeds of six kochia populations, three GR and three non-GR (susceptible), were placed on filter paper in dishes treated with varying doses of glyphosate. After 7-d incubation, seedlings with a true leaf (i.e., not cotyledons) at 25 mg L-1 glyphosate accurately distinguished GR from susceptible kochia seed lots. This bioassay can facilitate more timely confirmation of GR kochia, thereby aiding resistant weed management.

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