Effect of Propionibacterium freudenreichii on ruminal fermentation patterns, methane production and lipid biohydrogenation of beef finishing diets containing flaxseed oil in a rumen simulation technique.

Meale, S.J., Ding, S., He, M.L., Dugan, M.E.R, Ribeiro Jr, G.O., Alazzeh, A.Y., Holo, H., Harstad, O.M., McAllister, T.A., and Chaves, A.V. (2014). "Effect of Propionibacterium freudenreichii on ruminal fermentation patterns, methane production and lipid biohydrogenation of beef finishing diets containing flaxseed oil in a rumen simulation technique.", Canadian Journal of Animal Science, 94(4), pp. 685-695. doi : 10.4141/CJAS-2014-051  Access to full text

Abstract

The objectives of this study were to examine the effects of Propionibacterium freudenreichii (strain T54; PB) and flaxseed oil (FO) in a total mixed ration on ruminal fermentation, CH4 production and fatty acid biohydrogenation in two artificial rumens (RUSITEC). The experiment consisted of 8 d of adaptation and 12 d of sample collection with four replicate fermenters per treatment. Treatments were: (1) CON; (2) PB; (3) FO (60 g kg1 DM with autoclaved PB); (4) FOPB (60 g kg1 DM with PB). Disappearance of DM (g kg1 DM) and gas production (mL g1 DM) were not affected by treatment (P>0.05). Inclusion of FOPB increased (P=0.01) total volatile fatty acid (VFA) production (mmol d1), compared with CON and PB. The acetate:propionate ratio was reduced (P<0.001) in all treatments, compared with CON. Methane production (mL g1 DM or mL g1 DMD) was lowest (P<0.001) with PB (27.1%); however, FO (14.3%) and FOPB (19.3%) also reduced CH4 compared with CON. Fatty acid profiles for PB were similar (P>0.05) to CON for most fatty acids. Concentrations of 18:3n-3 were greater (P<0.001) in FO and FOPB in both digesta and effluent, compared with CON. Propionibacterium freudenreichii had very little effect on ruminal biohydrogenation, but reduced CH4 production under the current conditions as a result of increasing propionate production.

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