Complete genome sequence of three tomato ringspot virus isolates: evidence for reassortment and recombination.
Walker, M.C., Chisholm, J., Wei, T., Ghoshal, B., Saeed, H., Rott, M., and Sanfaçon, H. (2015). "Complete genome sequence of three tomato ringspot virus isolates: evidence for reassortment and recombination.", Archives of Virology, 160(2), pp. 543-547. doi : 10.1007/s00705-014-2240-y Access to full text
The genome sequence of tomato ringspot virus (ToRSV, a subgroup C nepovirus) is currently available for one raspberry isolate. In this study, we describe the complete genome sequence of three additional isolates from raspberry (Rasp1-2014), grapevine (GYV-2014) and prunus (13C280). The degree of nucleotide sequence identity shared between RNA1 and RNA2 in the 5′-terminal 900 nucleotides and 3′ untranslated region varied from 98-99 % (13C280, GYV-2014) to 80 % (Rasp1-2014). Phylogenetic studies revealed distinct origins for Rasp1-2014 RNA1 and RNA2, suggesting reassortment. Two recombination events were also identified in the 3′ UTR and 5′-terminal region of RNA1.
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