Assessment of a new Bacteroidales marker targeting North American Beaver (Castor Canadensis) fecal pollution by real-time PCR.

Romain, M., Zhang, Y., Zhang, Y., Tien, Y.C., Lapen, D.R., and Topp, E. (2013). "Assessment of a new Bacteroidales marker targeting North American Beaver (Castor Canadensis) fecal pollution by real-time PCR.", Journal of Microbiological Methods, 95(2), pp. 201-206. doi : 10.1016/j.mimet.2013.08.016  Access to full text

Abstract

In many settings wildlife can be a significant source of fecal pathogen input into surface water. The North-American beaver (Castor Canadensis) is a zoonotic reservoir for several human pathogens including Cryptosporidium spp. and Giardia spp. In order to specifically detect fecal pollution by beavers, we have developed and validated a beaver-specific Bacteroidales marker, designated Beapol01, based on the 16S rRNA gene. The marker is suitable for quantifying pollution using real-time PCR. The specificity and sensitivity of the marker was excellent, Beaver signal was detected in water of a mixed-activity watershed harbouring this rodent. Overall, Beapol01 will be useful for a better understanding of fecal source inputs in drainage basins inhabited by the beaver.

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