Effect of Emulsion Formulation of Sinorhizobium Meliloti and Pre-Inoculated Seeds on Alfalfa Nodulation and Growth: A Pouch Study.

John, R.P., Tyagi, R.D., Brar, S.K., Prévost, D., and Surampalli, R.Y. (2013). "Effect of Emulsion Formulation of Sinorhizobium Meliloti and Pre-Inoculated Seeds on Alfalfa Nodulation and Growth: A Pouch Study.", Journal of Plant Nutrition, 36(2), pp. 231-242. doi : 10.1080/01904167.2012.739243  Access to full text

Abstract

Emulsion formulation found to be optimal for survival of Sinorhizobium meliloti and the efficiency was tested for the nodulation on alfalfa. The emulsion formulation can be diluted up to 105 to 107 times to achieve maximum nodulation index (18) and to get maximum growth of the plant (height and dry weight). Emulsion can be applied on seed to make pre-inoculated seeds and storage at 4°C for two months provides more than 105 CFU/seeds (minimum standard requirement is 103 CFU/seeds). Nodulation test proved that both minimum and maximum requirement provide highest nodulation index and almost similar growth effect after 28 days of growth in pouches.

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