Monitoring Current Season Spread of Potato virus Y in Potato Fields Using ELISA and Real-time RT-PCR.

Fageria, M.S., Singh, M., Nanayakkara, U.N., Pelletier, Y., Nie, X., and Wattie, D. (2013). "Monitoring Current Season Spread of Potato virus Y in Potato Fields Using ELISA and Real-time RT-PCR.", Plant Disease, 97(5), pp. 641-644. doi : 10.1094/PDIS-03-12-0283-RE  Access to full text

Abstract

The current season spread of Potato virus Y (PVY) was investigated in New Brunswick, Canada, in eleven potato fields planted with six different cultivars in 2009 and 2010. One hundred plants selected from each field were monitored for current season PVY infections using enzyme-linked immunosorbent assay (ELISA) and real-time reverse-transcription-polymerase chain reaction assay (real-time RT-PCR). Average PVY incidence in fields increased from 0.6% in 2009 and 2% in 2010 in the leaves to 20.3% in 2009 and 21.9% in 2010 in the tubers at the time of harvest. In individual fields, PVY incidence in tubers reached as high as 37% in 2009 and 39% in 2010 at the time of harvest. Real-time RT-PCR assay detected more samples with PVY from leaves than did ELISA. A higher number of positive samples was also detected with real-time RT-PCR from growing tubers compared to the leaves collected from the same plant at the same sampling time. PVY incidence determined from the growing tubers showed a significant positive correlation with the PVY incidence of tubers after harvest. Pre-harvest testing provides another option to growers to either top-kill the crop immediately to secure the seed market when the PVY incidence is low or to leave the tubers to develop further for table or processing purposes when incidence of PVY is high.

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