Fruit Differential Protein Patterns in Strawberry Cultivars Susceptible or Resistant to Gray Mold.
Wang, Q., Dubé, C., Gagnon, C., Gleddie, S.C., Hao, Y.-J., and Khanizadeh, S. (2013). "Fruit Differential Protein Patterns in Strawberry Cultivars Susceptible or Resistant to Gray Mold.", Archives of Phytopathology and Plant Protection, 46(7), pp. 813-824. doi : 10.1080/03235408.2012.752619 Access to full text
The total proteins of four strawberry cultivars with differing resistance and/or susceptibility to gray mould were extracted by two different methods, phenol and sodium dodecyl sulfate (SDS). Compared to phenol extraction, SDS extraction produced higher protein yields but poor-quality protein bands with a high degree of streaking, indicating possible contamination. In contrast, the total proteins obtained by phenol extraction could be used in two-dimensional polyacrylamide gel electrophoresis (2-DE) because of their higher quality, and the protein spots were well separated for all tested genotypes. From all the cultivars studied in this research, we obtained 89 differential (unmatched) protein spots that may be related to fruit disease resistance or susceptibility. Using the SPSS 11.0 software, we obtained a cluster of the four strawberry cultivars. The cultivars ‘APF029-4’ and ‘SJ8976-1’ were in the same subcluster with the highest Pearson correlation coefficient (0.705). Afterwards, they were amalgamated into the second group with ‘Joliette,’ a disease-resistant cultivar, which means that they might have a similar resistance to gray mold.
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